Traditional Chinese medicine Tongxie Yaofang treating irritable bowel syndrome with diarrhea and type 2 diabetes mellitus in rats with liver-depression and spleen-deficiency: A preliminary study.
Traditional Chinese medicine Tongxie Yaofang treating irritable bowel syndrome with diarrhea and type 2 diabetes mellitus in rats with liver-depression and spleen-deficiency: A preliminary study.
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中药痛泻要方治疗肝郁脾虚型肠易激综合征腹泻并2型糖尿病大鼠的初步研究
DOI：
10.3389/fnut.2022.968930
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发表时间：
2022
影响因子：
5
通讯作者：
Tao, Wenhua
中科院分区：
文献类型：
--
作者：
Xu, Weidong;Zhang, Zhiyi;Lu, Ye;Li, Mengxi;Li, Jiayao;Tao, Wenhua
痛泻要方是由白术、白芍、陈皮、防风四味中药组成的治疗肠易激综合征（IBS）的中成药。然而，需要证明该配方改善D-IBS和T2 DM。本研究旨在通过动物模型观察通心泄肺方对D-IBS和T2 DM大鼠炎症的治疗作用。本研究采用高脂饲料、迷走神经和番泻叶灌胃建立大鼠靶疾病模型。分别采用H&E染色、ELISA和免疫组织化学方法检测肠道炎症、主要炎症因子和肠道通透性蛋白。本研究还利用Western blot来发现潜在的炎症靶点。本研究结果表明，通心泄肺方能降低肝郁脾虚型D-IBS和T2 DM大鼠血清TNF-α、IL-1β、IL-6水平，升高IL-10水平，提示炎症反应得到控制。染色分析还显示动物模型的疾病状态改善。此外，还检测到肠道渗透性相关蛋白claudin-1的有效反弹。此外，通心泻肺方可能通过rage通路对大鼠D-IBS和T2 DM有治疗作用。
Tongxie Yaofang (TXYF), a Traditional Chinese Medicine (TCM) with four components as follows: Rhizoma Atractylodis Macrocephalae (baizhu), Radix Paeoniae Alba (baishao), Pericarpium Citri Reticulatae (chenpi) and Radix Saposhnikovia Divaricata (fangfeng), benefits irritable bowel syndrome (IBS). Nonetheless, proofs of this formula ameliorating D-IBS and T2DM are required. This research aimed at investigating the efficacy of TXYF in treating inflammation in rats with D-IBS and T2DM using animal models. In this study, gavage with high-fat diet, fasciculation, and senna was given to develop rat models with target diseases. To determine intestinal inflammations, major inflammatory factors, and intestinal permeability proteins, H&E staining, ELISA, and immunohistochemistry methods were employed, respectively. This study also utilized Western blot to discover potential inflammatory targets. Results of this research illustrates that TXYF treatment reduced the level of TNF-α, IL-1β, and IL-6, and raised the IL-10 concentration in liver-depressed spleende ficient rats with D-IBS and T2DM, indicating controlled inflammatory reactions. Staining analysis also showed improved disease states of animal models. Furthermore, efficient rebounds of claudin-1, an intestinal permeability-associated protein, were detected. Moreover, TXYF may treat D-IBS and T2DM in rats via the rage pathway.
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影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
影响因子：
3.1
作者：
Gulcan, Erim;Taser, Figen;Alcelik, Aytekin
通讯作者：
Alcelik, Aytekin
评估血清白介素-10水平作为慢性牙周炎和2型糖尿病的血糖改变的预测指标。
影响因子：
--
作者：
Acharya AB;Thakur S;Muddapur MV
通讯作者：
Muddapur MV
影响因子：
8
作者：
Body-Malapel, M.;Djouina, M.;Vignal, C.
通讯作者：
Vignal, C.
影响因子：
6.6
作者：
Inai, T;Kobayashi, J;Shibata, Y
通讯作者：
Shibata, Y