Molecular analysis of the blood meals and bacterial communities of bed bugs (Cimex lectularius L.) to assess interactions with alternative hosts.
Molecular analysis of the blood meals and bacterial communities of bed bugs (Cimex lectularius L.) to assess interactions with alternative hosts.
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DOI：
10.1007/s00436-021-07079-x
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发表时间：
2021-04
影响因子：
2
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中科院分区：
文献类型：
--
作者：
常见臭虫（Cimex lectularius L.）是存在于地球仪的城市环境中的吸血害虫。人们普遍认为，它们对人类有强烈的宿主偏好。然而，有记录的C。温带温带关于常见臭虫在住宅环境中以替代宿主为食的频率，几乎没有信息，但了解这种现象对虫害管理和公共卫生都有影响。在这里，我们检查了C的队列。从美国新泽西州的13个不同住所收集的温带风虱，已知这些住所同时被家鼠（小家鼠）侵染。宿主特异性定量聚合酶链反应（qPCR）用于确定是否从小鼠中采集血餐，而16S rRNA基因扩增子测序用于筛选臭虫是否存在人畜共患细菌病原体。我们没有发现任何证据表明我们收集的臭虫以老鼠为食。此外，昆虫窝藏不包括已知的人类病原体的寄生细菌群落。然而，宿主特异性qPCR在一个住宅的臭虫池中检测到猫DNA，这表明与家养宠物的相互作用应进一步研究。虽然在这项研究中的采样是有限的，本文所述的方法将是有用的床虱和替代血餐主机之间的相互作用的额外的研究。
Common bed bugs (Cimex lectularius L.) are hematophagous pests present in urban environments across the globe. It is widely established that they have a strong host preference for humans. However, there are records of C. lectularius feeding upon a range of mammalian and avian hosts, including rodents, in the field. There is little information available about how frequently common bed bugs feed on alternative hosts in residential settings, but understanding this phenomenon has implications for both management of infestations and public health. Here, we examined cohorts of C. lectularius collected from 13 different dwellings in the state of New Jersey, USA, that were known to be simultaneously infested with house mice (Mus musculus domesticus). Host-specific quantitative polymerase chain reaction (qPCR) was used to determine if blood meals were taken from mice, while 16S rRNA gene amplicon sequencing was used to screen the bed bugs for the presence of zoonotic bacterial pathogens. We found no evidence that any of the bed bugs we collected fed on mice. Furthermore, the insects harbored depauperate bacterial communities that did not include known human pathogens. However, host-specific qPCR detected feline DNA in a pool of bed bugs from one dwelling, suggesting that interaction with domestic pets should be further investigated. Although sampling in this study was limited, the approach described herein will be useful for additional studies of the interactions between bed bugs and alternative blood meal hosts.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
2.1
作者：
Kjos SA;Marcet PL;Yabsley MJ;Kitron U;Snowden KF;Logan KS;Barnes JC;Dotson EM
通讯作者：
Dotson EM
影响因子：
2
作者：
Balvin, Ondrej;Munclinger, Pavel;Vilimova, Jitka
通讯作者：
Vilimova, Jitka
影响因子：
3.8
作者：
Leulmi, Hamza;Bitam, Idir;Parola, Philippe
通讯作者：
Parola, Philippe