Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra.
Development of a quantitative preclinical screening model for implant osseointegration in rat tail vertebra.
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DOI：
10.1007/s00784-018-2661-1
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发表时间：
2019-07
影响因子：
3.4
通讯作者：
Varga G
中科院分区：
文献类型：
--
作者：
Farkasdi S;Pammer D;Rácz R;Hriczó-Koperdák G;Szabó BT;Dobó-Nagy C;Kerémi B;Blazsek J;Cuisinier F;Wu G;Varga G
功能性牙齿替换和骨再生是现代牙科日常实践的一部分，但仍然缺乏重复性良好且相对便宜的实验模型。我们的目标是开发一种新的小动物模型，利用多种评估方案的组合来监测骨整合。在 Wistar 大鼠的 C4 和 C5 椎骨之间切割尾巴后，使用手术导板将平行壁、无螺纹的服装制成的植入物置于与其纵轴平行的尾巴中心。术后 4 至 16 周，对钛种植体进行骨整合跟踪，应用轴向拔除力，共振频率分析作为功能测试，组织形态计量学和显微 CT 作为结构评估。在功能测试中，我们观察到两种方法都适合检测骨整合随时间的增加，但拉出技术的灵敏度（大约增加五倍，标准误差相当低）远高于共振频率分析。在结构评估中，通过组织形态计量学测量的检测到的骨植入物接触值的变化（产量增加 1.5 倍，数据变化较小）比基于显微 CT 的评估更可靠，可筛选骨与植入物之间的接触发展。我们的结果证明尾椎骨整合模型可用于植入物与骨整合的临床前评估。生物力学和结构测试的结合提供了一个可重复性良好的小动物系统，适合研究各种植入材料和表面处理的整合。
Functional tooth replacement and bone regeneration are parts of the daily practice in modern dentistry, but well-reproducible and relatively inexpensive experimental models are still missing. We aimed to develop a new small animal model to monitor osseointegration utilizing the combination of multiple evaluation protocols. After cutting the tail between the C4 and C5 vertebrae in Wistar rats, costume made, parallel walled, non-threaded implants were placed into the center of the tail parallel with its longitudinal axis using a surgical guide. Osseointegration of the titanium implants was followed between 4 and 16 weeks after surgery applying axial extraction force, and resonance frequency analysis as functional tests, and histomorphometry and micro-CT as structural evaluations. In functional tests, we observed that both methods are suitable for the detection of the time-dependent increase in osseointegration, but the sensitivity of the pull-out technique (an approximately five times increase with rather low standard error) was much higher than that of the resonance frequency analysis. In structural evaluations, changes in the detected bone implant contact values measured by histomorphometry (yielding 1.5 times increase, with low variations of data) were more reliable than micro-CT based evaluations to screen the developments of contact between bone and implant. Our results provide evidence that the caudal vertebrae osseointegration model is useful for the preclinical evaluation of implant integration into the bone. The combination of the biomechanical and structural tests offers a well-reproducible small animal system that can be suitable for studying the integration of various implant materials and surface treatments.
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影响因子：
4.3
作者：
Berglundh, T;Abrahamsson, I;Lindhe, J
通讯作者：
Lindhe, J
DOI：
10.1002/ar.20748
发表时间：
2009-01-01
影响因子：
2
作者：
Haga, Maiko;Fujii, Noritaka;Maeda, Takeyasu
通讯作者：
Maeda, Takeyasu
影响因子：
0.9
作者：
Goelzer, Juliana Goncalves;Avelar, Rafael Linard;Machado, Rosilene Andrea
通讯作者：
Machado, Rosilene Andrea
影响因子：
4.3
作者：
Ito, Yoshihiro;Sato, Daisuke;Kasugai, Shohei
通讯作者：
Kasugai, Shohei
影响因子：
4.6
作者：
BRANEMARK, PI
通讯作者：
BRANEMARK, PI