Telomerase activity and telomere length in Daphnia.
Telomerase activity and telomere length in Daphnia.
复制标题
洗劫纳米的端粒酶活性和端粒长度。
DOI：
10.1371/journal.pone.0127196
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Patel RC
中科院分区：
文献类型：
--
作者：
Schumpert C;Nelson J;Kim E;Dudycha JL;Patel RC
端粒由短的重复序列组成，对基因组的稳定性是必不可少的，并已被研究与细胞衰老和衰老有关。端粒酶是一种将端粒重复序列添加到染色体末端的酶，对于维持端粒的整体长度是必不可少的。哺乳动物体细胞中端粒酶活性的缺乏导致端粒随着每次细胞复制事件而逐渐缩短。哺乳动物在胚胎和幼年阶段表现出很高的细胞增殖率，而在成体和衰老阶段体细胞增殖很少。细胞衰老的端粒假说认为，端粒是有丝分裂的内部时钟，端粒长度的侵蚀会导致细胞衰老，最终导致细胞死亡。在这份报告中，我们检测了水蚤的端粒酶活性、加工能力和端粒长度，这是一种在其一生中不断生长的有机体。与昆虫相似，水蚤端粒重复序列被确定为TTAGG，并在端粒酶活性测定中产生具有5个核苷酸周期的端粒酶产物。我们研究了两种紧密相关的生态型水蚤的端粒酶功能和端粒长度，这两种生态型的水蚤寿命不同，它们的寿命是短的和长的。我们的结果表明，在短命的杜氏杆菌中，端粒长度、端粒酶活性或端粒酶活性没有随年龄而下降。相反，在长期存活的肺吸虫中，观察到端粒长度、端粒酶活性和加工能力随年龄的显著下降。虽然提供了第一个关于水蚤端粒和端粒酶活性的报告，但我们的结果也表明，除了端粒缩短之外的其他机制可能是导致Daphnia Pulex寿命惊人缩短的原因。
Telomeres, comprised of short repetitive sequences, are essential for genome stability and have been studied in relation to cellular senescence and aging. Telomerase, the enzyme that adds telomeric repeats to chromosome ends, is essential for maintaining the overall telomere length. A lack of telomerase activity in mammalian somatic cells results in progressive shortening of telomeres with each cellular replication event. Mammals exhibit high rates of cell proliferation during embryonic and juvenile stages but very little somatic cell proliferation occurs during adult and senescent stages. The telomere hypothesis of cellular aging states that telomeres serve as an internal mitotic clock and telomere length erosion leads to cellular senescence and eventual cell death. In this report, we have examined telomerase activity, processivity, and telomere length in Daphnia, an organism that grows continuously throughout its life. Similar to insects, Daphnia telomeric repeat sequence was determined to be TTAGG and telomerase products with five-nucleotide periodicity were generated in the telomerase activity assay. We investigated telomerase function and telomere lengths in two closely related ecotypes of Daphnia with divergent lifespans, short-lived D. pulex and long-lived D. pulicaria. Our results indicate that there is no age-dependent decline in telomere length, telomerase activity, or processivity in short-lived D. pulex. On the contrary, a significant age dependent decline in telomere length, telomerase activity and processivity is observed during life span in long-lived D. pulicaria. While providing the first report on characterization of Daphnia telomeres and telomerase activity, our results also indicate that mechanisms other than telomere shortening may be responsible for the strikingly short life span of D. pulex.
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DOI：
10.1038/nrg3246
发表时间：
2012-10
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1161/atvbaha.109.185546
发表时间：
2009-06-01
影响因子：
8.7
作者：
Haendeler, Judith;Droese, Stefan;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
影响因子：
--
作者：
Herbert, Brittney-Shea;Shay, Jerry W;Wright, Woodring E
通讯作者：
Wright, Woodring E
影响因子：
4
作者：
Ahmed, Shaheda;Passos, Joao F.;Saretzki, Gabriele
通讯作者：
Saretzki, Gabriele
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL