circRNA expression patterns and circRNA-miRNA-mRNA networks during CV-A16 infection of SH-SY5Y cells.
circRNA expression patterns and circRNA-miRNA-mRNA networks during CV-A16 infection of SH-SY5Y cells.
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DOI：
10.1007/s00705-021-05190-z
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发表时间：
2021-11
影响因子：
2.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Hu Y;Yang R;Zhao W;Liu C;Tan Y;Pu D;Song J;Zhang Y
柯萨奇病毒A16型（CV-A16）引起了世界范围内婴儿和学龄前儿童手足口病（HFMD）的流行。环状RNA（CircRNA）是一类非编码RNA分子，参与病毒性感染性疾病的发生发展。尽管circRNA的功能已经是一个大量研究的主题，但它们在CV-A16感染中的作用仍然不清楚。在这项研究中，使用下一代测序技术研究了CV-A16对细胞circRNA转录组的病毒效应。结果表明，在感染后0、12和24 h分别鉴定出8726、8611和6826个circRNA。此外，发现1769和1192 circRNA差异表达在感染后12和24小时分别。将常见差异表达的circRNA进行功能注释分析，发现差异表达circRNA的亲本基因可能与病毒感染过程相关，尤其是GO分析中的“免疫系统过程”和KEGG分析中的“趋化因子和细胞因子信号通路介导的炎症”。随后，构建了circRNA-miRNA-mRNA调控网络，富集分析确定hsa_circ_0004447/hsa-miR-942- 5 p/MMP 2、hsa_circ_0078617/hsa-miR-6780 b-5 p/MMP 2和hsa_circ_0078617/hsa-miR-5196- 5 p/MMP 2调控轴是CV-A16感染进展过程中的重要网络。最后，选择了六个失调的circRNA进行验证，并验证与测序结果一致。考虑到所有这些结果，据我们所知，这项研究是第一个全面概述CV-A16感染诱导的circRNA，这项研究表明富集的circRNA和circRNA相关的竞争性内源性RNA（ceRNA）网络参与了CV-A16感染的调控，从而有助于阐明CV-A16-宿主相互作用的机制。在线版本包含补充材料，可通过10.1007/s 00705 -021-05190-z获得。
Coxsackievirus A16 (CV-A16) has caused worldwide epidemics of hand, foot, and mouth disease (HFMD) in infants and preschool children. Circular RNAs (circRNAs), a class of noncoding RNA molecules, participate in the progression of viral infectious diseases. Although the function of circRNAs has been a heavily researched topic, their role in CV-A16 infection is still unclear. In this study, the viral effects of CV-A16 on the cellular circRNA transcriptome were investigated using next-generation sequencing technology. The results showed that a total of 8726, 8611, and 6826 circRNAs were identified at 0, 12, and 24 h postinfection, respectively. Moreover, it was found that 1769 and 1192 circRNAs were differentially expressed in at 12 and 24 h postinfection, respectively. The common differentially expressed circRNAs were used for functional annotation analysis, and it was found that the parent genes of differentially expressed circRNAs might be associated with the viral infection process, especially the “Immune system process” in GO analysis and the “Inflammation mediated by chemokine and cytokine signaling pathway” in KEGG analysis. Subsequently, circRNA-miRNA-mRNA regulatory networks were constructed, and the hsa_circ_0004447/hsa-miR-942-5p/MMP2, hsa_circ_0078617/hsa-miR-6780b-5p/MMP2 and hsa_circ_0078617/hsa-miR-5196-5p/MMP2 regulatory axes were identified by enrichment analysis as important networks during the progression of CV-A16 infection. Finally, six dysregulated circRNAs were selected for validation and were verified to be consistent with the sequencing results. Considering all of these results, to the best of our knowledge, this study is the first to present a comprehensive overview of circRNAs induced by CV-A16 infection, and this research demonstrated that a network of enriched circRNAs and circRNA-associated competitive endogenous RNAs (ceRNAs) is involved in the regulation of CV-A16 infection, thereby helping to elucidate the mechanisms underlying CV-A16-host interactions. The online version contains supplementary material available at 10.1007/s00705-021-05190-z.
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DOI：
10.19540/j.cnki.cjcmm.20171106.012
发表时间：
2018-02-01
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
Gao, Jia-Liang;Chen, Guang;Wang, Jie
通讯作者：
Wang, Jie
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
影响因子：
5.6
作者：
Hu, Wei;Han, Qing;Wang, Li
通讯作者：
Wang, Li
影响因子：
0.5
作者：
Luplertlop, Natthanej;Suwanmanee, San;Poovorawan, Yong
通讯作者：
Poovorawan, Yong
影响因子：
8.7
作者：
Getts DR;Chastain EM;Terry RL;Miller SD
通讯作者：
Miller SD