Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses.
Transcriptional landscapes of de novo root regeneration from detached Arabidopsis leaves revealed by time-lapse and single-cell RNA sequencing analyses.
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通过延时和单细胞RNA测序分析揭示了拟南芥离体叶片从头根再生的转录景观。
DOI：
10.1016/j.xplc.2022.100306
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发表时间：
2022-07-11
影响因子：
10.5
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Liu, Wu;Zhang, Yuyun;Fang, Xing;Tran, Sorrel;Zhai, Ning;Yang, Zhengfei;Guo, Fu;Chen, Lyuqin;Yu, Jie;Ison, Madalene S.;Zhang, Teng;Sun, Lijun;Bian, Hongwu;Zhang, Yijing;Yang, Li;Xu, Lin
关键词：
拟南芥离体叶片可再生不定根，为研究新生根再生（de novo root regeneration， DNRR）提供了平台。然而，DNRR的综合转录框架仍然难以捉摸。在这里，我们提供了DNRR中从伤口反应到根器官发生的转录组重编程的高分辨率景观，并显示了DNRR中涉及的关键因素。对叶片脱落后12小时内整个叶片的延时RNA测序（RNA-seq）显示，茉莉酸盐、乙烯和活性氧（ROS）通路在损伤反应中迅速激活。遗传分析证实乙烯和ROS可能作为损伤信号促进DNRR。接下来，在离体叶片脱落后5 d内进行延时rna测序，揭示了在不定根过程中，离体叶片损伤区参与器官发生、损伤诱导再生和资源分配的基因的激活情况。遗传研究表明，控制地上器官的叶片- on - petiole1 /2、控制根系器官发生的PLETHORA3/5/7和控制伤口诱导再生的乙烯反应因子115参与了DNRR的调控。此外，单细胞RNA-seq数据揭示了不定根过程中离体叶片受伤区域的基因表达模式。总的来说，我们的研究不仅提供了转录组工具，而且揭示了与离体拟南芥叶片DNRR相关的关键因素。这项研究提供了一个高分辨率的转录组图谱，描述了离体拟南芥叶片新生根再生过程中的伤口反应和根器官发生。ROS和乙烯作为伤口信号促进再生，BOP1/2、PLT3/5/7和ERF115是调控根器官发生的关键基因。
Detached Arabidopsis thaliana leaves can regenerate adventitious roots, providing a platform for studying de novo root regeneration (DNRR). However, the comprehensive transcriptional framework of DNRR remains elusive. Here, we provide a high-resolution landscape of transcriptome reprogramming from wound response to root organogenesis in DNRR and show key factors involved in DNRR. Time-lapse RNA sequencing (RNA-seq) of the entire leaf within 12 h of leaf detachment revealed rapid activation of jasmonate, ethylene, and reactive oxygen species (ROS) pathways in response to wounding. Genetic analyses confirmed that ethylene and ROS may serve as wound signals to promote DNRR. Next, time-lapse RNA-seq within 5 d of leaf detachment revealed the activation of genes involved in organogenesis, wound-induced regeneration, and resource allocation in the wounded region of detached leaves during adventitious rooting. Genetic studies showed that BLADE-ON-PETIOLE1/2, which control aboveground organs, PLETHORA3/5/7, which control root organogenesis, and ETHYLENE RESPONSE FACTOR115, which controls wound-induced regeneration, are involved in DNRR. Furthermore, single-cell RNA-seq data revealed gene expression patterns in the wounded region of detached leaves during adventitious rooting. Overall, our study not only provides transcriptome tools but also reveals key factors involved in DNRR from detached Arabidopsis leaves. This study provides a high-resolution transcriptome atlas that profiles wound response and root organogenesis during de novo root regeneration from detached Arabidopsis leaves. ROS and ethylene serve as the wound signals to promote regeneration, and BOP1/2, PLT3/5/7, and ERF115 are key genes that regulate root organogenesis.
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