Characterization of the Nrt2.6 gene in Arabidopsis thaliana: a link with plant response to biotic and abiotic stress.
Characterization of the Nrt2.6 gene in Arabidopsis thaliana: a link with plant response to biotic and abiotic stress.
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DOI：
10.1371/journal.pone.0042491
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Daniel-Vedele F
中科院分区：
文献类型：
--
作者：
Dechorgnat J;Patrit O;Krapp A;Fagard M;Daniel-Vedele F
拟南芥的高亲和力硝酸盐运输系统涉及一个基因和可能的七个基因，分别来自NRT1和NRT2家族。其中，NRT2.1、NRT2.2、NRT2.4和NRT2.7蛋白具有转运硝酸盐的功能，定位于质膜或液泡膜上。NRT2.1、NRT2.2和NRT2.4在根细胞对土壤溶液中硝酸盐的吸收中起作用，而NRT2.7负责种子液泡中的硝酸盐负荷。我们已经对该家族的第三个成员--NRT2.6基因进行了功能鉴定。NRT2.6在大多数植物器官中弱表达，在营养器官中的表达高于生殖器官。与其他NRT2成员不同的是，NRT2.6的表达不是通过限制而是由高氮水平诱导的，并且在nrt2.6-1突变体中没有发现与硝酸盐相关的表型。一直以来，该基因的过度表达未能弥补nrt2.1-nrt2.2双突变体的硝酸盐吸收缺陷。NRT2.6的表达是由植物病原菌淀粉欧文氏杆菌诱导的。有趣的是，NRT2.6表达降低的植物对病原菌攻击的耐受性较低。研究发现，NRT2.6的表达与淀粉芽孢杆菌侵染和氧化还原活性除草剂甲基紫精处理引起的ROS积累之间存在相关性，提示NRT2.6活性与ROS的产生可能存在联系。
The high affinity nitrate transport system in Arabidopsis thaliana involves one gene and potentially seven genes from the NRT1 and NRT2 family, respectively. Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. We have undertaken the functional characterization of a third member of the family, the NRT2.6 gene. NRT2.6 was weakly expressed in most plant organs and its expression was higher in vegetative organs than in reproductive organs. Contrary to other NRT2 members, NRT2.6 expression was not induced by limiting but rather by high nitrogen levels, and no nitrate-related phenotype was found in the nrt2.6-1 mutant. Consistently, the over-expression of the gene failed to complement the nitrate uptake defect of an nrt2.1-nrt2.2 double mutant. The NRT2.6 expression is induced after inoculation of Arabidopsis thaliana by the phytopathogenic bacterium Erwinia amylovora. Interestingly, plants with a decreased NRT2.6 expression showed a lower tolerance to pathogen attack. A correlation was found between NRT2.6 expression and ROS species accumulation in response to infection by E. amylovora and treatment with the redox-active herbicide methyl viologen, suggesting a probable link between NRT2.6 activity and the production of ROS in response to biotic and abiotic stress.
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