Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen.
Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen.
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DOI：
10.3389/fpls.2018.01278
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发表时间：
2018
影响因子：
5.6
通讯作者：
Urwin PE
中科院分区：
文献类型：
--
作者：
Hoysted GA;Bell CA;Lilley CJ;Urwin PE
植物同时遭受来自地上和地下的多种生物威胁。通常对这些害虫和/或病原体进行个体研究，并且地上害虫对地下病原体的影响尚不清楚。对马铃薯植物（Solanum tuberosum L.）的根分泌物进行了分析，以表征韧皮部吸食草食动物（Myzus persicae）和静止的内寄生线虫（Globodera pallida）之间自上而下的植物介导的相互作用。增加蚜虫 M. persicae 的接种量会减少马铃薯植株的根质量。在 28 天的时间内，从这些根部收集的分泌物诱导的 G. pallida 卵孵化的第二阶段幼体的孵化率明显低于未受感染的对照植物的孵化率。孵化抑制与蚜虫接种量呈显着正相关。用未受感染的马铃薯植株的根部分泌物处理后，孵化率下降部分恢复，表明对孵化的影响是可逆的，但无法完全恢复。与对照相比，受蚜虫侵染的马铃薯植株根系分泌物中的葡萄糖和果糖含量降低，这些糖被发现可诱导 G. pallida 孵化，但程度与马铃薯根系分泌物 (PRE) 不同。用糖补充受蚜虫侵染的 PRE 并没有恢复处理的孵化潜力，这表明其他化合物在卵孵化中发挥着重要作用。在密切相关的马铃薯胞囊线虫 Globodera rostochiensis 暴露于宿主根部分泌物后上调的第一个基因 Neprilysin-1，被证实在暴露于 PRE 后在 G. pallida 胞囊中上调，并且通过糖处理也上调。在用感染大量蚜虫的马铃薯植株的根分泌物处理的包囊中观察到 Gpa-nep-1 的上调显着减少。我们的数据表明，马铃薯植株的蚜虫侵染会影响根部分泌物的成分，从而影响 G. pallida 卵的孵化和基因表达。这项工作表明，地上害虫可以间接影响根际，并揭示了控制经济上重要的地下病原体的二次效应。
Plants suffer multiple, simultaneous biotic threats from both above and below ground. These pests and/or pathogens are commonly studied on an individual basis and the effects of above-ground pests on below-ground pathogens are poorly defined. Root exudates from potato plants (Solanum tuberosum L.) were analyzed to characterize the top-down plant-mediated interactions between a phloem-sucking herbivore (Myzus persicae) and a sedentary, endoparasitic nematode (Globodera pallida). Increasing inocula of the aphid, M. persicae, reduced the root mass of potato plants. Exudates collected from these roots induced significantly lower hatching of second-stage juveniles from G. pallida eggs over a 28-day period, than those from uninfested control plants. Inhibition of hatch was significantly positively correlated with size of aphid inoculum. Diminished hatching was partially recovered after treatment with root exudate from uninfested potato plants indicating that the effect on hatching is reversible but cannot be fully recovered. Glucose and fructose content was reduced in root exudates from aphid-infested potato plants compared to controls and these sugars were found to induce hatching of G. pallida, but not to the same degree as potato root exudates (PRE). Supplementing aphid-infested PRE with sugars did not recover the hatching potential of the treatment, suggesting that additional compounds play an important role in egg hatch. The first gene upregulated in the closely related potato cyst nematode Globodera rostochiensis post-exposure to host root exudate, Neprilysin-1, was confirmed to be upregulated in G. pallida cysts after exposure to PRE and was also upregulated by the sugar treatments. Significantly reduced upregulation of Gpa-nep-1 was observed in cysts treated with root exudates from potato plants infested with greater numbers of aphids. Our data suggest that aphid infestation of potato plants affects the composition of root exudates, with consequential effects on the hatching and gene expression of G. pallida eggs. This work shows that an above-ground pest can indirectly impact the rhizosphere and reveals secondary effects for control of an economically important below-ground pathogen.
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DOI：
10.1017/s0022149x00031485
发表时间：
1940-01-01
期刊：
JOUR HELMINTHOL
影响因子：
--
作者：
FENWICK, D. W.
通讯作者：
FENWICK, D. W.
影响因子：
2.8
作者：
Foster, SP;Denholm, I;Devonshire, AL
通讯作者：
Devonshire, AL
影响因子：
2.3
作者：
Hol, W. H. Gera;De Boer, Wietse;Termorshuizen, Aad J.;Meyer, Katrin M.;Schneider, Johannes H. M.;Van der Putten, Wim H.;Van Dam, Nicole M.
通讯作者：
Van Dam, Nicole M.
影响因子：
5.6
作者：
Hoysted GA;Lilley CJ;Field KJ;Dickinson M;Hartley SE;Urwin PE
通讯作者：
Urwin PE
影响因子：
12.3
作者：
Cotton JA;Lilley CJ;Jones LM;Kikuchi T;Reid AJ;Thorpe P;Tsai IJ;Beasley H;Blok V;Cock PJ;Eves-van den Akker S;Holroyd N;Hunt M;Mantelin S;Naghra H;Pain A;Palomares-Rius JE;Zarowiecki M;Berriman M;Jones JT;Urwin PE
通讯作者：
Urwin PE