Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance.
Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance.
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DOI：
10.1038/s42003-021-02372-y
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发表时间：
2021-07-15
影响因子：
5.9
通讯作者：
Pan D
中科院分区：
文献类型：
--
作者：
Ostadhossein F;Moitra P;Altun E;Dutta D;Sar D;Tripathi I;Hsiao SH;Kravchuk V;Nie S;Pan D
牙菌斑是生物膜，通过产酸细菌的脱矿作用引起龋齿。这些细菌驻留在保护套内，这使得任何治愈治疗都具有挑战性。我们提出了一种无杀虫剂的策略，通过在生物膜的酸性环境中发挥作用的工程化簇状碳点纳米颗粒来破坏生物膜。对变形链球菌成熟生物膜的体外和离体研究显示，>90%的生物膜抑制与纳米颗粒与细菌膜的接触介导的相互作用、过量活性氧物质的产生和DNA片段化有关。体内实验表明，纳米粒能有效抑制S.变种人重要的是，牙齿微生物群的16S rRNA分析表明，细菌物种的多样性和丰富性在纳米颗粒治疗后没有发生实质性变化。总的来说，这项研究提供了一种安全有效的方法来减少牙齿生物膜的形成，而不会破坏口腔的生态平衡。Ostadhossein等人提出了一种无杀虫剂的策略，通过利用设计用于在生物膜的酸性环境中发挥作用的纳米颗粒来破坏生物膜。Phophonium离子官能化的碳点纳米簇在pH敏感的聚合物壳显示牙齿生物膜抑制，而不破坏口腔的生态平衡。
Dental plaques are biofilms that cause dental caries by demineralization with acidogenic bacteria. These bacteria reside inside a protective sheath which makes any curative treatment challenging. We propose an antibiotic-free strategy to disrupt the biofilm by engineered clustered carbon dot nanoparticles that function in the acidic environment of the biofilms. In vitro and ex vivo studies on the mature biofilms of Streptococcus mutans revealed >90% biofilm inhibition associated with the contact-mediated interaction of nanoparticles with the bacterial membrane, excessive reactive oxygen species generation, and DNA fragmentation. An in vivo examination showed that these nanoparticles could effectively suppress the growth of S. mutans. Importantly, 16S rRNA analysis of the dental microbiota showed that the diversity and richness of bacterial species did not substantially change with nanoparticle treatment. Overall, this study presents a safe and effective approach to decrease the dental biofilm formation without disrupting the ecological balance of the oral cavity. Ostadhossein et al. propose an antibiotic-free strategy to disrupt the biofilm by utilizing nanoparticles designed to function in the acidic environment of the biofilms. Phophonium ions functionalised carbon dot nanoclusters in a pH-sensitive polymer shell display dental biofilm inhibition without disrupting the ecological balance of the oral cavity.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
17.1
作者：
Jian, Hong-Jyuan;Wu, Ren-Siang;Huang, Chih-Ching
通讯作者：
Huang, Chih-Ching
DOI：
10.1073/pnas.1010341107
发表时间：
2010-12-21
影响因子：
11.1
作者：
Beyth, Nurit;Yudovin-Farber, Ira;Weiss, Ervin I.
通讯作者：
Weiss, Ervin I.
影响因子：
9.5
作者：
Kim, Domyoung;Kwon, Seok-Joon;Dordick, Jonathan S.
通讯作者：
Dordick, Jonathan S.
影响因子：
4.9
作者：
Hope, CK;Wilson, M
通讯作者：
Wilson, M