Presence of DNA from Chlamydia-like organisms in the nasal cavities of grey seal pups (Halichoerus grypus) and three different substrates present in a breeding colony.
Presence of DNA from Chlamydia-like organisms in the nasal cavities of grey seal pups (Halichoerus grypus) and three different substrates present in a breeding colony.
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DOI：
10.1186/s12917-021-03032-3
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发表时间：
2021-10-13
影响因子：
2.6
通讯作者：
Longbottom D
中科院分区：
文献类型：
--
作者：
Dagleish MP;Flockhart AF;Baily JL;Hall AJ;Simpson TI;Longbottom D
衣原体样生物（CLO）已被发现存在于许多环境生态位，包括人类污水和农业径流，以及在世界各地的一些水生物种。因此，监测它们在哨兵野生物种中的存在，可能有助于评估海洋食物网因生境丧失、污染和疾病而产生的更广泛的健康状况。我们使用鼻拭子活（n = 42）和死亡（n = 50）前断奶的灰海豹幼崽和不同的纳塔尔基板（n = 8）从离岸岛屿没有牲畜和永久人类居住的样本，以确定是否CLO DNA存在于这些哺乳动物，并确定可能的来源。我们从32/92（34.7%）活（n = 17）和死（n = 15）海豹幼崽的鼻拭子中回收了CLO DNA，这些海豹幼崽与目前公认的属于三个衣原体家族的物种最接近：副衣原体科（n = 22），弹状衣原体科（n = 6）和Simkaniaceae（n = 3）。所有DNA阳性沉积物样品（n = 7）与弹状衣原体科聚类。在活幼仔与死幼仔中未发现CLO DNA的回收率存在差异，表明微生物是寄生的，但无法确定其作为机会性继发病原体的潜力。这是首次在海洋哺乳动物中发现CLO DNA。这一发现证明了在英国海岸和世界其他地区的其他海豹种群中进行进一步调查的必要性，以确定这一发现是独特的还是比这些数据所显示的更常见。还需要进行进一步调查，以确定它们是否作为纯粹的水生生物存在，或者它们是否也可能是海豹中的机会致病菌，并调查可能的来源，包括它们是否源于人类废物和农业径流等人为影响。
Chlamydia-like organisms (CLO) have been found to be present in many environmental niches, including human sewage and agricultural run-off, as well as in a number of aquatic species worldwide. Therefore, monitoring their presence in sentinel wildlife species may be useful in assessing the wider health of marine food webs in response to habitat loss, pollution and disease. We used nasal swabs from live (n = 42) and dead (n = 50) pre-weaned grey seal pups and samples of differing natal substrates (n = 8) from an off-shore island devoid of livestock and permanent human habitation to determine if CLO DNA is present in these mammals and to identify possible sources. We recovered CLO DNA from 32/92 (34.7%) nasal swabs from both live (n = 17) and dead (n = 15) seal pups that clustered most closely with currently recognised species belonging to three chlamydial families: Parachlamydiaceae (n = 22), Rhabdochlamydiaceae (n = 6), and Simkaniaceae (n = 3). All DNA positive sediment samples (n = 7) clustered with the Rhabdochlamydiaceae. No difference was found in rates of recovery of CLO DNA in live versus dead pups suggesting the organisms are commensal but their potential as opportunistic secondary pathogens could not be determined. This is the first report of CLO DNA being found in marine mammals. This identification warrants further investigation in other seal populations around the coast of the UK and in other areas of the world to determine if this finding is unique or more common than shown by this data. Further investigation would also be warranted to determine if they are present as purely commensal organisms or whether they could also be opportunistic pathogens in seals, as well as to investigate possible sources of origin, including whether they originated as a result of anthropogenic impacts, including human waste and agricultural run-off.
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DOI：
10.1038/ismej.2017.95
发表时间：
2017-10
期刊：
The ISME journal
影响因子：
--
作者：
Collingro A;Köstlbacher S;Mussmann M;Stepanauskas R;Hallam SJ;Horn M
通讯作者：
Horn M
影响因子：
3.7
作者：
Mitura, Agata;Niemczuk, Krzysztof;Szymanska-Czerwinska, Monika
通讯作者：
Szymanska-Czerwinska, Monika
影响因子：
2.4
作者：
Bossart, G. D.
通讯作者：
Bossart, G. D.
影响因子：
5.1
作者：
Corsaro, Daniele;Feroldi, Veronica;Greub, Gilbert
通讯作者：
Greub, Gilbert
影响因子：
4.3
作者：
Barkallah, M.;Jribi, H.;Gdoura, R.
通讯作者：
Gdoura, R.