Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis
Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis
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抗天蚕素 B 副猪嗜血杆菌的基因表达谱
DOI：
10.1159/000362277
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Feiqun Xue
中科院分区：
文献类型：
--
作者：
Xungang Ku;Qigai He;Huanchun Chen;Feiqun Xue
合成设计的抗菌肽（AMPs）在治疗细菌感染方面具有替代抗生素的潜力。然而，微生物对抗菌肽的耐药性已有报道，但对这种耐药性的潜在机制知之甚少。天然存在的AMP cecropin B （CB）破坏革兰氏阴性菌的阴离子细胞膜。在本研究中，将副猪嗜血杆菌SH0165暴露于一系列CB浓度下，诱导其产生CB抗性（CBR）。获得抗乙肝副猪嗜血杆菌菌株CBR30和CBR30-50。SH0165和CBR30的生长曲线显示，CBR30的生长速率低于SH0165。透射电镜结果显示，抗cb的CBR30和CBR30-50的细胞膜比SH0165光滑。与SH0165相比，微阵列检测到耐药CBR30基因中有257个基因上调，254个基因下调，覆盖20个同源群（COGs）簇（>变化1.5倍，p < 0.05）。60个基因在CBR30-50中受到影响，覆盖18个COGs，其中28个基因上调，32个基因下调。在COG功能分类下，抗cb CBR30和CBR30-50中大部分受影响的基因属于无机离子转运、氨基酸转运和代谢范畴。通过实时定量反转录PCR验证微阵列结果。本研究为了解副猪嗜血杆菌对炭疽杆菌耐药的分子机制提供了有益的指导。
Synthetically designed antimicrobial peptides (AMPs) present the potential of replacing antibiotics in the treatment of bacterial infections. However, microbial resistance to AMPs has been reported and little is known regarding the underlying mechanism of such resistance. The naturally occurring AMP cecropin B (CB) disrupts the anionic cell membranes of Gram-negative bacteria. In this study, CB resistance (CBR) was induced in Haemophilusparasuis SH0165 by exposing it to a series of CB concentrations. The CB-resistant H.parasuis strains CBR30 and CBR30-50 were obtained. The growth curves of SH0165 and CBR30 showed that CBR30 displayed lower growth rates than SH0165. The result of transmission electron microscopy showed cell membranes of the CB-resistant CBR30 and CBR30-50 were smoother than SH0165. Microarrays detected 257 upregulated and 254 downregulated genes covering 20 clusters of orthologous groups (COGs) of the CB-resistant CBR30 compared with SH0165 (>1.5-fold change, p < 0.05). Sixty genes were affected in CBR30-50 covering 18 COGs, with 28 upregulated and 32 downregulated genes. Under the COG function classification, the majority of affected genes in the CB-resistant CBR30 and CBR30-50 belong to the category of inorganic ion transport, amino acid transport, and metabolism. The microarray results were validated by real-time quantitative reverse transcription PCR. This study may provide useful guidance for understanding the molecular mechanism underlying H.parasuis resistance to CB.
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影响因子：
0.7
作者：
A. Wissing;J. Nicolet;P. Boerlin
通讯作者：
A. Wissing;J. Nicolet;P. Boerlin
影响因子：
3
作者：
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影响因子：
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作者：
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通讯作者：
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2.6
作者：
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通讯作者：
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影响因子：
2.5
作者：
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通讯作者：
Blecha, Frank