Synergistic Effects of DNA Structure for Ultrasensitive Detecting OTA in Grains
Synergistic Effects of DNA Structure for Ultrasensitive Detecting OTA in Grains
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DNA 结构对超灵敏检测谷物中 OTA 的协同作用
DOI：
10.1007/s12161-021-02060-x
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发表时间：
2021-06
影响因子：
2.9
通讯作者：
Xiaohui Xiong
中科院分区：
文献类型：
--
作者：
Wei Yuan;Lixia Lu;Yichen Lu;Xiong Xiong;Yi Li;Xiaowen Cui;Yuanjian Liu;Xiaohui Xiong
Ochratoxin A (OTA) is considered as one of the most hazardous mycotoxins commonly occurring in grains. Thus, the monitoring of OTA is extremely significant. Herein, a synergistic strategy to detect OTA in grains based on the combination of DNA tetrahedron-structured aptamer (DTA) and hybridization chain reaction (HCR) was proposed. Briefly, DTA was assembled on the gold electrode. In the presence of OTA, the trigger DNA on the top of the DTA was exposed to unfold the biotin-modified hairpin structure DNA to HCR. It could conjugate plentiful streptavidin-labeled horseradish peroxidase (SA-HRP) to form polyaniline (PANI), which was regarded as electrochemical active molecule to detect OTA. In this way, the synergistic effect of DTA and HCR not only can provide stable probe structure to improve the recognition rate of OTA, but also can supply a large number of templates to synthesize PANI under mild conditions. As a result, the biosensor has a good analytical performance for OTA in a linear range of 0.05 to 50 ng mL−1, with a detection limit of 0.017 ng mL−1. The accuracy of sensor is similar with high-performance liquid chromatography (HPLC) analysis.
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影响因子：
8.4
作者：
Huan Zhang;Xinxin Liu;Chun-yang Zhang;Yi Xu;Jing Su;Xiaoliang Lu;Jiye Shi;Lihua Wang;M. Landry;Yin‐Min Zhu;Min Lv;Xianqiang Mi
通讯作者：
Huan Zhang;Xinxin Liu;Chun-yang Zhang;Yi Xu;Jing Su;Xiaoliang Lu;Jiye Shi;Lihua Wang;M. Landry;Yin‐Min Zhu;Min Lv;Xianqiang Mi
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DOI：
10.1016/j.snb.2020.128375
发表时间：
2020-10
期刊：
Sensors and Actuators B: Chemical
影响因子：
--
作者：
Xi-Luan Yan;Xin-Xin Xue;Jing Luo;Yu-Ting Jian;Li Tong;Xiang-Juan Zheng
通讯作者：
Xiang-Juan Zheng
影响因子：
6.1
作者：
Albani L Gonzalez;Valeria A. Lozano;G. M. Escandar;Manuel A. Bravo
通讯作者：
Albani L Gonzalez;Valeria A. Lozano;G. M. Escandar;Manuel A. Bravo
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影响因子：
2.9
作者：
Qu, Jianwen;Xie, Huijuan;Jiang, Wenxiao
通讯作者：
Jiang, Wenxiao
影响因子：
8.8
作者：
Hao, Liangwen;Chen, Jing;Xiong, Yonghua
通讯作者：
Xiong, Yonghua