Ex Vivo and In Vivo Properties of an Injectable Hydrogel Derived From Acellular Ear Cartilage Extracellular Matrix.
Ex Vivo and In Vivo Properties of an Injectable Hydrogel Derived From Acellular Ear Cartilage Extracellular Matrix.
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来自脱细胞耳软骨细胞外基质的可注射水凝胶的体外和体内特性
DOI：
10.3389/fbioe.2021.740635
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发表时间：
2021
影响因子：
5.7
通讯作者：
Lu Y
中科院分区：
文献类型：
--
作者：
Gong D;Yu F;Zhou M;Dong W;Yan D;Zhang S;Yan Y;Wang H;Tan Y;Chen Y;Feng B;Fu W;Fu Y;Lu Y
细胞外基质（ECM）水凝胶提供了诸如可注射性、填充不规则形状空间的能力以及天然基质的足够生物活性的优点。在这项研究中，我们开发了脱细胞软骨细胞外基质（dcECM）水凝胶从猪耳通过酶消化的主要方法，并验证了良好的生物相容性的dcECM水凝胶输送软骨细胞，并在体内形成皮下软骨。采用扫描电子显微镜和凝胶化动力学浊度法对dcECM水凝胶的材料性质和凝胶化动力学进行了表征。通过体外软骨细胞培养评价了水凝胶的生物相容性。为了进一步探索体内dcECM水凝胶，将由dcECM水凝胶和软骨细胞的混合物制成的移植物皮下注射到裸鼠中用于大体和组织学分析。dcECM水凝胶的结构和凝胶化动力学根据ECM浓度的变化而改变。10 mg/ml的dcECM水凝胶在体外可支持软骨细胞的粘附和增殖。在体内，移植后4周，各组均检测到软骨样组织，甲苯胺蓝、番红O和胶原II染色阳性，表明dcECM水凝胶凝胶化良好。而随着浓度的增加，由10 mg/ml dcECM水凝胶移植物形成的组织工程软骨在重量、体积、胶原和糖胺聚糖（GAG）含量方面上级1 mg/ml和5 mg/ml的dcECM水凝胶。在移植后8周，10 mg/ml的dcECM水凝胶移植物显示出与对照胶原I移植物非常相似的品质。体内培养12周后，组织学分析表明，10 mg/ml的dcECM水凝胶移植物与猪耳的正常软骨相似，这是来源组织。结论：dcECM水凝胶具有良好的组织工程生物材料的应用前景，可促进耳软骨的再生和修复损伤。
Extracellular matrix (ECM) hydrogels provide advantages such as injectability, the ability to fill an irregularly shaped space, and the adequate bioactivity of native matrix. In this study, we developed decellularized cartilage ECM (dcECM) hydrogels from porcine ears innovatively via the main method of enzymatic digestion and verified good biocompatible properties of dcECM hydrogels to deliver chondrocytes and form subcutaneous cartilage in vivo. The scanning electron microscopy and turbidimetric gelation kinetics were used to characterize the material properties and gelation kinetics of the dcECM hydrogels. Then we evaluated the biocompatibility of hydrogels via the culture of chondrocytes in vitro. To further explore the dcECM hydrogels in vivo, grafts made from the mixture of dcECM hydrogels and chondrocytes were injected subcutaneously in nude mice for the gross and histological analysis. The structural and gelation kinetics of the dcECM hydrogels altered according to the variation in the ECM concentrations. The 10 mg/ml dcECM hydrogels could support the adhesion and proliferation of chondrocytes in vitro. In vivo, at 4 weeks after transplantation, cartilage-like tissues were detected in all groups with positive staining of toluidine blue, Safranin O, and collagen II, indicating the good gelation of dcECM hydrogels. While with the increasing concentration, the tissue engineering cartilages formed by 10 mg/ml dcECM hydrogel grafts were superior in weights, volumes, collagen, and glycosaminoglycan (GAG) content compared to the dcECM hydrogels of 1 mg/ml and 5 mg/ml. At 8 weeks after grafting, dcECM hydrogel grafts at 10 mg/ml showed very similar qualities to the control, collagen I grafts. After 12 weeks of in vivo culture, the histological analysis indicated that 10 mg/ml dcECM hydrogel grafts were similar to the normal cartilage from pig ears, which was the source tissue. In conclusion, dcECM hydrogel showed the promising potential as a tissue engineering biomaterial to improve the regeneration and heal injuries of ear cartilage.
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影响因子：
5.3
作者：
Nooeaid P;Salih V;Beier JP;Boccaccini AR
通讯作者：
Boccaccini AR
影响因子：
14
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
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影响因子：
14
作者：
Liu, Kai;Zhou, Guang Dong;Cao, Yilin
通讯作者：
Cao, Yilin
影响因子：
0.1
作者：
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通讯作者：
Fu, Yao
影响因子：
3.1
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DeQuach JA;Lin JE;Cam C;Hu D;Salvatore MA;Sheikh F;Christman KL
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Christman KL