Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses.
Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses.
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DOI：
10.3390/molecules28041890
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发表时间：
2023-02-16
期刊：
影响因子：
4.6
通讯作者：
Im, Eunok
中科院分区：
文献类型：
--
作者：
Jeong, Sihyun;Kim, Yuju;Park, Soyeong;Lee, Doyeon;Lee, Juho;Hlaing, Shwe Phyu;Yoo, Jin-Wook;Rhee, Sang Hoon;Im, Eunok
植物乳杆菌是一种新型益生菌，可用于治疗癌症、特应性皮炎、炎症性肠病和感染等多种疾病。在这项研究中，我们研究了植物乳杆菌代谢产物的抗癌作用的潜在机制。我们培养了植物乳杆菌细胞以获得其代谢产物，创建了几种稀释液，并将这些溶液用于治疗人结肠癌Caco-2细胞。我们的结果表明，植物乳杆菌代谢产物稀释10%会降低细胞存活率，并减少自噬相关蛋白的表达。此外，我们发现植物乳杆菌代谢产物和氯喹(一种已知的自噬抑制剂)联合处理在细胞毒性和自噬相关蛋白表达下调方面具有协同效应。总之，我们发现植物乳杆菌的代谢产物与氯喹具有协同作用，可以杀死Caco-2细胞并下调自噬相关蛋白的表达，这表明自噬而不是细胞凋亡参与了它们的细胞毒作用。因此，本研究通过抑制自噬为新的治疗方法提供了新的见解。
Lactobacillus plantarum (L. plantarum) is a probiotic that has emerged as novel therapeutic agents for managing various diseases, such as cancer, atopic dermatitis, inflammatory bowel disease, and infections. In this study, we investigated the potential mechanisms underlying the anticancer effect of the metabolites of L. plantarum. We cultured L. plantarum cells to obtain their metabolites, created several dilutions, and used these solutions to treat human colonic Caco-2 cells. Our results showed a 10% dilution of L. plantarum metabolites decreased cell viability and reduced the expression of autophagy-related proteins. Moreover, we found co-treatment with L. plantarum metabolites and chloroquine, a known autophagy inhibitor, had a synergistic effect on cytotoxicity and downregulation of autophagy-related protein expression. In conclusion, we showed the metabolites from the probiotic, L. plantarum, work synergistically with chloroquine in killing Caco-2 cells and downregulating the expression of autophagy-related proteins, suggesting the involvement of autophagy, rather than apoptosis, in their cytotoxic effect. Hence, this study provides new insights into new therapeutic methods via inhibiting autophagy.
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DOI：
10.1016/j.bbrc.2016.04.098
发表时间：
2016-05-20
影响因子：
3.1
作者：
Kawaguchi, Moemi;Aoki, Shigeki;Ito, Kousei
通讯作者：
Ito, Kousei
影响因子：
1.5
作者：
Hasannejad-Bibalan, Meysam;Mojtahedi, Ali;Talebi, Malihe
通讯作者：
Talebi, Malihe
影响因子：
13.3
作者：
Chung, Ki Wung;Kim, Kyung Mok;Chung, Hae Young
通讯作者：
Chung, Hae Young
影响因子：
20.3
作者：
Goussetis, Dennis J.;Gounaris, Elias;Platanias, Leonidas C.
通讯作者：
Platanias, Leonidas C.
影响因子：
2.7
作者：
Hiraishi, Nobuhiro;Kanmura, Shuji;Ido, Akio
通讯作者：
Ido, Akio