Cold exposure promotes obesity and impairs glucose homeostasis in mice subjected to a high‑fat diet.
Cold exposure promotes obesity and impairs glucose homeostasis in mice subjected to a high‑fat diet.
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寒冷暴露会促进高脂肪饮食小鼠的肥胖并损害葡萄糖稳态
DOI：
10.3892/mmr.2018.9382
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发表时间：
2018-10
影响因子：
3.4
通讯作者：
Lin S
中科院分区：
文献类型：
--
作者：
Zhu P;Zhang ZH;Huang XF;Shi YC;Khandekar N;Yang HQ;Liang SY;Song ZY;Lin S
寒冷暴露被认为是一种压力，对身体有各种不利影响。本研究旨在探讨慢性冷暴露对高脂饮食（HFD）小鼠摄食量、体重、血糖水平和中枢能量平衡调节通路的影响。将C57 BL/6小鼠分成两组，用标准食物或HFD喂养。每组中的一半小鼠暴露于冰冷的水中，每天1小时，持续7周，而对照组暴露于室温。与对照组相比，慢性每日冷暴露显著增加了HFD喂养小鼠的能量摄入、体重和血糖水平。此外，最后一次冷暴露后1小时，与未冷暴露的HFD小鼠相比，喂食HFD的小鼠中央杏仁核中的c-fos免疫反应性显着增加，表明该脑区的神经元激活。值得注意的是，这些c-fos神经元共表达神经肽Y（NPY），食欲肽水平显着增加，在中央杏仁核的冷暴露小鼠与对照组相比。值得注意的是，冷暴露显着降低了下丘脑腹内侧核中的促凋亡脑源性神经营养因子（BDNF）信使RNA（mRNA）水平，并增加了室旁核中的生长激素释放激素（GHRH）mRNA。HFD小鼠的中枢杏仁核中的NPY能神经元被慢性冷暴露激活，通过神经元通路减少BDNF和增加GHRH mRNA表达，可能有助于肥胖和葡萄糖稳态受损的发展。
Cold exposure is considered to be a form of stress and has various adverse effects on the body. The present study aimed to investigate the effects of chronic daily cold exposure on food intake, body weight, serum glucose levels and the central energy balance regulatory pathway in mice fed with a high-fat diet (HFD). C57BL/6 mice were divided into two groups, which were fed with a standard chow or with a HFD. Half of the mice in each group were exposed to ice-cold water for 1 h/day for 7 weeks, while the controls were exposed to room temperature. Chronic daily cold exposure significantly increased energy intake, body weight and serum glucose levels in HFD-fed mice compared with the control group. In addition, 1 h after the final cold exposure, c-fos immunoreactivity was significantly increased in the central amygdala of HFD-fed mice compared with HFD-fed mice without cold exposure, indicating neuronal activation in this brain region. Notably, 61% of these c-fos neurons co-expressed the neuropeptide Y (NPY), and the orexigenic peptide levels were significantly increased in the central amygdala of cold-exposed mice compared with control mice. Notably, cold exposure significantly decreased the anorexigenic brain-derived neurotropic factor (BDNF) messenger RNA (mRNA) levels in the ventromedial hypothalamic nucleus and increased growth hormone releasing hormone (GHRH) mRNA in the paraventricular nucleus. NPY-ergic neurons in the central amygdala were activated by chronic cold exposure in mice on HFD via neuronal pathways to decrease BDNF and increase GHRH mRNA expression, possibly contributing to the development of obesity and impairment of glucose homeostasis.
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影响因子：
5.2
作者：
Kuo LE;Czarnecka M;Kitlinska JB;Tilan JU;Kvetnanský R;Zukowska Z
通讯作者：
Zukowska Z
影响因子：
64.5
作者：
Fedorenko A;Lishko PV;Kirichok Y
通讯作者：
Kirichok Y
影响因子：
2.9
作者：
Liu X;Zhu Z;Kalyani M;Janik JM;Shi H
通讯作者：
Shi H
影响因子：
2.5
作者：
Lee, Seul Ki;Ryu, Pan Dong;Lee, So Yeong
通讯作者：
Lee, So Yeong
影响因子：
4
作者：
Eshkevari, Ladan;Permaul, Eva;Mulroney, Susan E.
通讯作者：
Mulroney, Susan E.