Systematic analysis of goal-related movement sequences during maternal behaviour in a female mouse model for Rett syndrome.
Systematic analysis of goal-related movement sequences during maternal behaviour in a female mouse model for Rett syndrome.
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DOI：
10.1111/ejn.15327
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Krishnan K
中科院分区：
文献类型：
--
作者：
Stevenson PK;Casenhiser DM;Lau BYB;Krishnan K
啮齿动物水坝寻找并将分散的幼崽带回巢穴（幼崽回收），这是母性护理的一个重要方面。对构成整个行为序列的目标相关运动的动态序列的系统分析对于理解潜在的神经生物学至关重要，但目前还没有得到很好的表征。在这里，我们对两种基因型的异亲雌性小鼠（代孕小鼠或 Sur）进行了三天的分析； Mecp2杂合子 (Het)，Rett 综合征的雌性小鼠模型及其野生型 (WT) 兄弟姐妹。我们分析了 CBA/CaJ 和 C57BL/6J WT 替代品以进行品系内比较。使用 DataVyu 软件手动执行不同阶段的逐帧分析。我们之前通过延迟指数和错误等端点分析表明，代理 Het 在幼崽检索方面效率低下。在这里，WT 的搜索、小狗方法和成功检索的顺序在几天内简化，而 Het 则表现出这种模式的变化。 Het 和 WT 之间的目标相关运动在其他阶段相似，表明在幼犬检索阶段 Het 中环境驱动的非典型模式。我们将幼犬近端接近和梳理幼犬视为幼犬和 Het 之间的非典型触觉互动。每日分析显示，不同基因型和品系的个体动物与目标相关的运动存在动态变化。总体而言，我们的方法 1) 突出了不同日期个体小鼠的自然变化，2) 建立了一个“黄金标准”手动整理的数据集，以帮助使用机器学习方法构建行为库，3) 表明雷特综合征雌性小鼠模型中的非典型触觉感觉处理和可能的回归。
Rodent dams seek and gather scattered pups back to the nest (pup retrieval), an essential aspect of maternal care. Systematic analysis of the dynamic sequences of goal-related movements that comprise the entire behavioral sequence, which would be ultimately essential for understanding the underlying neurobiology, is not well-characterized. Here, we present such analysis across three days in alloparental female mice (Surrogates or Sur) of two genotypes; Mecp2Heterozygotes (Het), a female mouse model for Rett syndrome and their wild type (WT) siblings. We analyzed CBA/CaJ and C57BL/6J WT surrogates for within-strain comparisons. Frame-by-frame analysis over different phases was performed manually using DataVyu software. We previously showed that surrogate Het are inefficient at pup retrieval, by end-point analysis such as latency index and errors. Here, the sequence of searching, pup-approach and successful retrieval streamlines over days for WT, while Het exhibits variations in this pattern. Goal-related movements between Het and WT are similar in other phases, suggesting context-driven atypical patterns in Het during the pup retrieval phase. We identified proximal pup approach and pup grooming as atypical tactile interactions between pups and Het. Day-by-day analysis showed dynamic changes in goal-related movements in individual animals across genotypes and strains. Overall, our approach 1) highlights natural variation in individual mice on different days, 2) establishes a “gold-standard” manually curated dataset to help build behavioral repertoires using machine learning approaches, and 3) suggests atypical tactile sensory processing and possible regression in a female mouse model for Rett syndrome.
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影响因子：
5.3
作者：
Chong, Kelly K.;Anandakumar, Dakshitha B.;Liu, Robert C.
通讯作者：
Liu, Robert C.
影响因子：
2.2
作者：
Alsina-Llanes, Marcela;De Brun, Victoria;Olazabal, Daniel E.
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影响因子：
1.9
作者：
CALAMANDREI, G;KEVERNE, EB
通讯作者：
KEVERNE, EB
影响因子：
2.9
作者：
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通讯作者：
VOMSAAL, FS
影响因子：
7.4
作者：
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通讯作者：
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