Tissue-specific reductions in mitochondrial efficiency and increased ROS release rates during ageing in zebra finches, Taeniopygia guttata.
Tissue-specific reductions in mitochondrial efficiency and increased ROS release rates during ageing in zebra finches, Taeniopygia guttata.
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DOI：
10.1007/s11357-022-00624-1
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发表时间：
2023-02
期刊：
影响因子：
5.6
通讯作者：
Dawson, Neal J.
中科院分区：
文献类型：
--
作者：
Salmon, Pablo;Millet, Caroline;Selman, Colin;Monaghan, Pat;Dawson, Neal J.
线粒体功能障碍和氧化损伤一直被认为是动物衰老过程中至关重要的机制。然而，关于这是否涉及衰老过程中线粒体活性氧（ROS）的产生增加，存在相互矛盾的数据。我们采用高分辨率呼吸测定法和荧光测定法对飞行肌（胸大肌）和肝脏线粒体进行检测，同时检测年轻（3个月）和老年（4岁）斑胸草雀（Taeniopygia guttata）的线粒体功能和ROS（H2 O2）释放率。氧化磷酸化的呼吸能力在两个年龄组的组织中没有差异。肝脏线粒体的呼吸控制率（RCR）在年龄组之间也没有差异。然而，肌肉线粒体中的RCR在老年人中比年轻人低55%，这表明老年人的肌肉线粒体效率较低。有趣的是，这种观察到的肌肉RCR的降低几乎完全是由更高的线粒体LEAK状态呼吸驱动的。最大的线粒体ROS释放率被认为是更大的飞行肌肉（1.3倍）和肝脏（1.9倍）的老鸟。然而，虽然线粒体的最大ROS（H2 O2）释放速率随着年龄的增长而增加，但肝脏和肌肉组织的ROS释放量与年龄相关的增加比例大于肌肉。肌肉和肝脏组织之间与年龄相关的ROS释放速率增加的这种差异可能是由于老年鸟类肌肉中线粒体泄漏增加，而不是肝脏。这表明，与年龄相关的细胞功能变化似乎以组织特异性的方式发生在斑胸草雀中，飞行肌肉表现出与年龄相关的ROS释放增加最小化的迹象，可能会减少对老年人这一关键组织的损伤。在线版本包含补充材料，可通过10.1007/s11357-022-00624-1获得。
Mitochondrial dysfunction and oxidative damage have long been suggested as critically important mechanisms underlying the ageing process in animals. However, conflicting data exist on whether this involves increased production of mitochondrial reactive oxygen species (ROS) during ageing. We employed high‐resolution respirometry and fluorometry on flight muscle (pectoralis major) and liver mitochondria to simultaneously examine mitochondrial function and ROS (H2O2) release rates in young (3 months) and old (4 years) zebra finches (Taeniopygia guttata). Respiratory capacities for oxidative phosphorylation did not differ between the two age groups in either tissue. Respiratory control ratios (RCR) of liver mitochondria also did not differ between the age classes. However, RCR in muscle mitochondria was 55% lower in old relative to young birds, suggesting that muscle mitochondria in older individuals are less efficient. Interestingly, this observed reduction in muscle RCR was driven almost entirely by higher mitochondrial LEAK-state respiration. Maximum mitochondrial ROS release rates were found to be greater in both flight muscle (1.3-fold) and the liver (1.9-fold) of old birds. However, while maximum ROS (H2O2) release rates from mitochondria increased with age across both liver and muscle tissues, the liver demonstrated a proportionally greater age-related increase in ROS release than muscle. This difference in age-related increases in ROS release rates between muscle and liver tissues may be due to increased mitochondrial leakiness in the muscle, but not the liver, of older birds. This suggests that age-related changes in cellular function seem to occur in a tissue-specific manner in zebra finches, with flight muscle exhibiting signs of minimising age-related increase in ROS release, potentially to reduce damage to this crucial tissue in older individuals. The online version contains supplementary material available at 10.1007/s11357-022-00624-1.
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