Prevalence of Chromosomally Located bla(CTX-M-55) in Salmonella Typhimurium ST34 Isolates Recovered from a Tertiary Hospital in Guangzhou, China.
Prevalence of Chromosomally Located bla(CTX-M-55) in Salmonella Typhimurium ST34 Isolates Recovered from a Tertiary Hospital in Guangzhou, China.
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从中国广州一家三级医院回收的鼠伤寒沙门氏菌 ST34 分离株中染色体定位的 bla CTX-M-55 的患病率
DOI：
10.1128/spectrum.02771-21
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Li, Xiaoyan
中科院分区：
文献类型：
--
作者：
Zeng, Shihan;Zhuo, Zhenxu;Huang, Yulan;Luo, Jiajun;Feng, Yulian;Gong, Baiyan;Huang, Xiyi;Wu, Aiwu;Zhuo, Chao;Li, Xiaoyan
非伤寒沙门氏菌（NTS）是全球范围内引起胃肠道感染的最常见细菌之一。与此同时，CTX-M-55 ESBL阳性检出率在我国也逐渐上升。为了解携带blaCTX-M-55的非伤寒沙门氏菌（NTS）临床分离株的分子流行病学和基因组特征，从中国某三级医院收集了105株NTS分离株。进行抗菌药物敏感性试验以确定耐药表型。通过全基因组测序和生物信息学分析，确定了blaCTX-M-55基因的耐药基因、血清型、系统发育关系和遗传环境。结果表明，在22株头孢曲松耐药菌株中，blaCTX-M-55是14株菌株最常见的β-内酰胺酶基因，包括血清型S。鼠伤寒（10/14）、沙门氏菌（S.明斯特（2/14），S. Rissen（1/14）和S.圣保罗（1/14）。系统发育分析表明，10株blaCTX-M-55阳性的S.鼠伤寒ST 34分离株分为两个簇。聚类分析表明，各聚类中的菌株亲缘关系非常近（≤10个cgMLST位点）。blaCTX-M-55基因定位于10个分离株的染色体上，3个分离株的IncI 1质粒上，1个分离株的IncHI 2质粒上。因此，blaCTX-M-55基因主要定位于S.伤寒沙门氏菌ST 34菌株是NTS对头孢菌素类抗生素耐药的主要驱动力。因此，应密切关注携带blaCTX-M-55的S.必须仔细监测临床环境中的鼠伤寒沙门氏菌ST 34。重要性ESC是治疗NTS感染的首选。ESBLs和AmpC酶是ESCs耐药的最典型原因。近年来临床上CTX-M-55产ESBL阳性率逐渐增高。目前，对blaCTX-M-55阳性沙门氏菌的研究主要集中在食源性动物或环境中，对临床患者的研究较少。因此，本研究旨在鉴定携带blaCTX-M-55的NTS临床分离株的分子流行病学和基因组特征。结果表明，blaCTX-M-55基因主要定位于S.从化地区分离的鼠伤寒ST 34是NTS对头孢菌素类抗生素耐药的主要驱动力。因此，我们的工作强调了监测携带blaCTX-M-55的S.临床环境中的鼠伤寒沙门氏菌ST 34。
Nontyphoidal Salmonella (NTS) is one of the most prevalent bacterial causes of gastrointestinal infections worldwide. Meanwhile, the detection rate of CTX-M-55 ESBL-positive has increased gradually in China. To identify the molecular epidemiological and genomic characteristics of blaCTX-M-55-carrying nontyphoidal Salmonella (NTS) clinical isolates, a total of 105 NTS isolates were collected from a Chinese tertiary hospital. Antimicrobial susceptibility testing was performed to determine the resistance phenotype. Whole-genome sequencing and bioinformatics analysis were used to determine the antimicrobial resistance genes, serotypes, phylogenetic relationships, and the genetic environment of the blaCTX-M-55 gene. The results showed that among the 22 ceftriaxone resistant isolates, the blaCTX-M-55 was the most common β-Lactamase gene carried by 14 isolates, including serotypes S. Typhimurium (10/14), S. Muenster (2/14), S. Rissen (1/14), and S. Saintpaul (1/14). Phylogenetic analysis shows that 10 blaCTX-M-55-positive S. Typhimurium ST34 isolates were divided into two clusters. The genetic relationship of isolates in each cluster was very close (≤10 cgMLST loci). The blaCTX-M-55 gene was located on the chromosome in 10 isolates, on IncI1 plasmid in three isolates, and IncHI2 plasmid in one isolate. In conclusion, the blaCTX-M-55 gene, mainly located on the chromosome of S. Typhimurium ST34 isolates, was the main driving force associated with the resistance of NTS to cephalosporins. Therefore, close attention to the clonal dissemination of blaCTX-M-55-carrying S. Typhimurium ST34 in clinical settings must be monitored carefully. IMPORTANCE ESCs are the first choice for treating NTS infections. However, ESBLs and AmpC β-lactamases are the most typical cause for ESCs resistance. The CTX-M-55 ESBL-positive rate has gradually increased in the clinic in recent years. At present, the research about blaCTX-M-55-positive Salmonella mainly focuses on the foodborne animals or the environment while less on clinical patients. Thus, this study was carried out for identifying molecular epidemiological and genomic characteristics of blaCTX-M-55-carrying NTS clinical isolates. The results showed that the blaCTX-M-55 gene, mainly located on the chromosome of S. Typhimurium ST34 isolates from Conghua District, was the main driving force associated with the resistance of NTS to cephalosporins. Therefore, our work highlights the importance of monitoring the clonal dissemination of blaCTX-M-55-carrying S. Typhimurium ST34 in clinical settings.
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