Micropatterned multicolor dynamically adhesive substrates to control cell adhesion and multicellular organization.
Micropatterned multicolor dynamically adhesive substrates to control cell adhesion and multicellular organization.
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DOI：
10.1021/la404037s
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发表时间：
2014-02-11
期刊：
影响因子：
--
通讯作者：
Chen CS
中科院分区：
文献类型：
--
作者：
Rodriguez NM;Desai RA;Trappmann B;Baker BM;Chen CS
我们提出了一种新的技术来检查细胞间的相互作用和定向细胞迁移使用微图案基板的三个不同的区域：一个粘性区域，一个非粘性区域，和一个动态粘性区域切换通过添加可溶性因子的介质。结合微接触印刷与抗生物素蛋白-生物素捕获化学，我们图案的抗生物素蛋白，成为粘合剂通过捕获生物素化的纤连蛋白的非粘性区域。我们的策略克服了目前的双色动态粘合剂基板的几个限制，将第三，永久非粘性区域。具有三个空间上和功能上不同的区域允许实现更复杂的细胞共培养物的配置以及两个细胞群体之间的复杂界面几何形状，用于不同的异型细胞-细胞相互作用研究。我们现在可以实现空间控制的路径和方向的迁移，除了时间控制的迁移开始，使研究，更好地概括协调的多细胞迁移和组织在体外。我们证实，细胞行为是不变的捕获生物素化的纤连蛋白相比，打印纤连蛋白通过检查细胞的能力，蔓延，形成粘连，并迁移。我们证明了这种方法在迁移和细胞共培养的研究中的多功能性，并通过探测Notch-Delta aptacrine信号在图案化界面进一步突出其实用性。
We present a novel technique to examine cell–cell interactions and directed cell migration using micropatterned substrates of three distinct regions: an adhesive region, a nonadhesive region, and a dynamically adhesive region switched by addition of a soluble factor to the medium. Combining microcontact printing with avidin–biotin capture chemistry, we pattern nonadhesive regions of avidin that become adhesive through the capture of biotinylated fibronectin. Our strategy overcomes several limitations of current two-color dynamically adhesive substrates by incorporating a third, permanently nonadhesive region. Having three spatially and functionally distinct regions allows for the realization of more complex configurations of cellular cocultures as well as intricate interface geometries between two cell populations for diverse heterotypic cell–cell interaction studies. We can now achieve spatial control over the path and direction of migration in addition to temporal control of the onset of migration, enabling studies that better recapitulate coordinated multicellular migration and organization in vitro. We confirm that cellular behavior is unaltered on captured biotinylated fibronectin as compared to printed fibronectin by examining the cells’ ability to spread, form adhesions, and migrate. We demonstrate the versatility of this approach in studies of migration and cellular cocultures, and further highlight its utility by probing Notch–Delta juxtacrine signaling at a patterned interface.
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影响因子：
15
作者：
Jiang, XY;Ferrigno, R;Whitesides, GM
通讯作者：
Whitesides, GM
DOI：
10.1083/jcb.200510062
发表时间：
2006-07-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pirone DM;Liu WF;Ruiz SA;Gao L;Raghavan S;Lemmon CA;Romer LH;Chen CS
通讯作者：
Chen CS
影响因子：
3.9
作者：
Ostuni, E;Kane, R;Whitesides, GM
通讯作者：
Whitesides, GM
影响因子：
5.8
作者：
Li, JT;Caldwell, KD
通讯作者：
Caldwell, KD
影响因子：
1.4
作者：
Rogers, JA;Paul, KE;Whitesides, GM
通讯作者：
Whitesides, GM