Dually Fluorescent Sensing of pH and Dissolved Oxygen Using a Membrane Made from Polymerizable Sensing Monomers.
Dually Fluorescent Sensing of pH and Dissolved Oxygen Using a Membrane Made from Polymerizable Sensing Monomers.
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DOI：
10.1016/j.snb.2010.03.029
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发表时间：
2010-06-03
影响因子：
8.4
通讯作者：
Meldrum, Deirdre R.
中科院分区：
文献类型：
--
作者：
Tian, Yanqing;Shumway, Bradley R.;Youngbull, A. Cody;Li, Yongzhong;Jen, Alex K. -Y.;Johnson, Roger H.;Meldrum, Deirdre R.
采用热聚合方法和分别具有绿色和红色发射光谱的可聚合pH和氧气传感单体，以聚（甲基丙烯酸2-羟乙酯）-共聚（丙烯酰胺）作为基质，制备了新型pH、氧气及其双传感膜。将传感器接枝在丙烯酸酯改性石英玻璃上，并在不同的 pH 值、氧气浓度、离子强度、温度和细胞培养基下进行表征。 pH 和氧传感器使用相同的激发波长进行激发，并表现出良好分离的发射光谱。 pH 传感膜在 5.5 至 8.5 的 pH 范围内表现出良好的响应，对应于 6.9 至 7.1 之间的生物相关范围内的 pKa 值。氧传感膜对溶解氧表现出线性 Stern-Volmer 猝灭响应。由于传感膜是使用含传感部分的单体的热引发聚合来制备的，因此没有观察到传感器从膜浸出到缓冲液或介质中。这种有利的特性在一定程度上解释了传感器的生物相容性，在孵育 40 小时后对 HeLa 细胞没有明显的毒性。采用双传感膜同时测量pH值和溶解氧。测量结果与设定点值相关。
Using a thermal polymerization approach and polymerizable pH and oxygen sensing monomers with green and red emission spectra, respectively, new pH, oxygen, and their dual sensing membranes were prepared using poly(2-hydroxyethyl methacrylate)-co-poly(acrylamide) as a matrix. The sensors were grafted on acrylate-modified quartz glass and characterized under different pH values, oxygen concentrations, ion strengths, temperatures and cell culture media. The pH and oxygen sensors were excited using the same excitation wavelength and exhibited well-separated emission spectra. The pH-sensing films showed good response over the pH range 5.5 to 8.5, corresponding to pKa values in the biologically-relevant range between 6.9 and 7.1. The oxygen-sensing films exhibited linear Stern-Volmer quenching responses to dissolved oxygen. As the sensing membranes were prepared using thermally initiated polymerization of sensing moiety-containing monomers, no leaching of the sensors from the membranes to buffers or medium was observed. This advantageous characteristic accounts in part for the sensors' biocompatibility without apparent toxicity to HeLa cells after 40 hours incubation. The dual-sensing membrane was used to measure pH and dissolved oxygen simultaneously. The measured results correlated with the set-point values.
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DOI：
10.1039/a701130j
发表时间：
1997-06-01
期刊：
ANALYTICAL COMMUNICATIONS
影响因子：
--
作者：
Lee, SK;Okura, I
通讯作者：
Okura, I
影响因子：
8.4
作者：
Lee, Seungjoon;They, Bennett L.;Pishko, Michael V.
通讯作者：
Pishko, Michael V.
影响因子：
7.4
作者：
Borisov, S. M.;Nuss, G.;Klimant, I.
通讯作者：
Klimant, I.
影响因子：
4.2
作者：
Niu, CG;Gui, XQ;Yuan, XZ
通讯作者：
Yuan, XZ
影响因子：
8.4
作者：
DEMAS, JN;DEGRAFF, BA
通讯作者：
DEGRAFF, BA