N-glycans: phenotypic homology and structural differences between myocardial cells and induced pluripotent stem cell-derived cardiomyocytes.
N-glycans: phenotypic homology and structural differences between myocardial cells and induced pluripotent stem cell-derived cardiomyocytes.
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N-聚糖：心肌细胞和诱导多能干细胞衍生的心肌细胞之间的表型同源性和结构差异。
DOI：
10.1371/journal.pone.0111064
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sawa Y
中科院分区：
文献类型：
--
作者：
Kawamura T;Miyagawa S;Fukushima S;Yoshida A;Kashiyama N;Kawamura A;Ito E;Saito A;Maeda A;Eguchi H;Toda K;Lee JK;Miyagawa S;Sawa Y
细胞表面聚糖变化很大，这取决于细胞特性。我们假设，诱导多能干细胞（iPSC）上的聚糖表达可能会在向心肌表型分化的心肌分化过程中发生变化。从iPSC、iPSC衍生的心肌细胞（iPSC-CM）和原始C57 BL/6小鼠心肌（心脏）分离N-聚糖。通过基于HPLC洗脱时间和MALDI-TOF/MS光谱的映射技术分析它们的结构。分离了68种不同的N-聚糖;鉴定了其中60种N-聚糖的结构。iPSC上的高甘露糖型（未成熟）N-聚糖的量随着心肌分化而减少，但没有达到在心脏中观察到的低水平。我们观察到中性N-聚糖类似减少，岩藻糖基化或唾液酸N-聚糖增加。在iPSC-CM和心脏之间检测到一些结构差异。在iPSC-CM中没有检测到N-羟乙酰神经氨酸（NeuGc）结构，而心脏含有大量NeuGc结构，对应于胞苷一磷酸-N-乙酰神经氨酸羟化酶的表达。此外，在iPSC-CM中检测到在其他细胞中很少鉴定的几种含有Galα1-6 Gal的聚糖。在向心肌细胞分化的过程中，小鼠iPSC上的N-聚糖表达向心肌表型变化，留下NeuGc含量或Galα1-6 Gal结构的结构差异。需要进一步的研究来揭示iPSC-CM和心肌之间N-聚糖差异的意义。
Cell surface glycans vary widely, depending on cell properties. We hypothesized that glycan expression on induced pluripotent stem cells (iPSCs) might change during cardiomyogenic differentiation toward the myocardial phenotype. N-glycans were isolated from iPSCs, iPSC-derived cardiomyocytes (iPSC-CM), and original C57BL/6 mouse myocardium (Heart). Their structures were analyzed by a mapping technique based on HPLC elution times and MALDI-TOF/MS spectra. Sixty-eight different N-glycans were isolated; the structures of 60 of these N-glycans were identified. The quantity of high-mannose type (immature) N-glycans on the iPSCs decreased with cardiomyogenic differentiation, but did not reach the low levels observed in the heart. We observed a similar reduction in neutral N-glycans and an increase in fucosylated or sialyl N-glycans. Some structural differences were detected between iPSC-CM and Heart. No N-glycolyl neuraminic acid (NeuGc) structures were detected in iPSC-CM, whereas the heart contained numerous NeuGc structures, corresponding to the expression of cytidine monophosphate-N-acetylneuraminic acid hydroxylase. Furthermore, several glycans containing Galα1-6 Gal, rarely identified in the other cells, were detected in the iPSC-CM. The expression of N-glycan on murine iPSCs changed toward the myocardial phenotype during cardiomyogenic differentiation, leaving the structural differences of NeuGc content or Galα1-6 Gal structures. Further studies will be warranted to reveal the meaning of the difference of N-glycans between the iPSC-CM and the myocardium.
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