Clinical detection of precataractous lens protein changes using dynamic light scattering.
Clinical detection of precataractous lens protein changes using dynamic light scattering.
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DOI：
10.1001/archophthalmol.2008.507
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发表时间：
2008-12
影响因子：
--
通讯作者：
Ferris, Frederick L., III
中科院分区：
文献类型：
--
作者：
Datiles, Manuel B., III;Ansari, Rafat R.;Suh, Kwang I.;Vitale, Susan;Reed, George F.;Zigler, J. Samuel, Jr.;Ferris, Frederick L., III
使用动态光散射 (DLS) 临床评估早期白内障前晶状体蛋白变化。我们对 235 名年龄 7-86 岁受试者的 380 只眼睛进行了一项横断面研究，AREDS 临床晶状体核等级范围为 0-3.8。 DLS 装置用于评估 α-晶状体蛋白，这是一种分子伴侣蛋白，可结合其他受损的晶状体蛋白，防止它们聚集。结果测量是 α-晶状体蛋白指数 (ACI)，它是每个晶状体中未结合的 α-晶状体蛋白的测量值。确定了 ACI 与核不透明度增加和衰老之间的关联。随着晶状体核混浊程度的增加，ACI 显着降低（p<0.0001）。即使在与老化相关的临床透明镜片中，α-晶状体蛋白也会显着损失（p<0.0001）。测量的标准误差为3%。即使在临床透明的镜片中，DLS 也能在临床上检测到 α-晶状体蛋白的损失。 ACI 测量可能有助于识别白内障高风险患者，并作为临床晶状体研究的结果变量。我们的研究表明，ACI 可能是衡量晶状体中存在的保护性 α-晶状体蛋白分子伴侣储备的有用指标，类似于估计肾功能储备中的肌酐清除率。
To use Dynamic Light Scattering (DLS) to clinically assess early pre-cataractous lens protein changes. We performed a cross sectional study in 380 eyes of 235 subjects aged 7–86 years with AREDS clinical lens nuclear grades ranging from 0–3.8. A DLS device was used to assess α-crystallin, a molecular chaperone protein shown to bind other damaged lens proteins, preventing their aggregation. The outcome measure was the α-crystallin index (ACI), a measure of unbound α-crystallin in each lens. The association of ACI with increasing nuclear opacity and aging was determined. There was a significant decrease in ACI associated with increasing grades of lens nuclear opacity (p<0.0001). There are significant losses of α-crystallin even in clinically clear lenses associated with aging (p<0.0001). The standard error of measurement was 3%. DLS clinically detects loss of α-crystallin proteins even in clinically clear lenses. ACI measurements may be useful in identifying patients at high risk for developing cataract, and as an outcome variable in clinical lens studies. Our studies suggest that the ACI may be a useful measure of the protective α-crystallin molecular chaperone reserve present in a lens, analogous to creatinine clearance in estimating renal function reserve.
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影响因子：
3.4
作者：
Ansari, RR;Suh, KI;Sebag, J
通讯作者：
Sebag, J
影响因子：
3.4
作者：
Clark, JI;Livesey, JC;Steele, JE
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Steele, JE
影响因子：
3.4
作者：
Hanson, SRA;Hasan, A;Smith, JB
通讯作者：
Smith, JB
影响因子：
3.4
作者：
Hiraoka, T;Clark, JI;Thurston, GM
通讯作者：
Thurston, GM
影响因子：
2
作者：
DATILES, MB;FUKUI, H
通讯作者：
FUKUI, H