New Cell Lines Derived from European Tick Species.

New Cell Lines Derived from European Tick Species.
复制标题

来自欧洲蜱物种的新细胞系。

DOI:
10.3390/microorganisms10061086
复制
发表时间:
2022-05-25
期刊:
影响因子:
4.5
通讯作者:
Makepeace, Benjamin L.
Makepeace, Benjamin L.
中科院分区:
生物学3区
文献类型:
--
作者:
Bell-Sakyi, Lesley;Hartley, Catherine S.;Khoo, Jing Jing;Forth, Jan Hendrik;Palomar, Ana M.;Makepeace, Benjamin L.

文献摘要

参考文献

相似文献

蜱细胞系是研究蜱及其传播的病原体的重要工具。在这里,我们报告了来自德国和西班牙的Argas,Dermacentor,Hyalomma,Ixodes和Rhipicephalus属的欧洲蜱的10个新细胞系的建立。对于每种细胞系,提供了用于生成原代培养物的方法、细胞的形态学描述和通过部分16S rRNA基因测序进行的种属确认。对两种新的蓖麻硬蜱细胞系和同一物种的三种现有细胞系的进一步分子分析揭示了来自相同或附近地点收集的蜱的细胞系之间的遗传变异。总的来说,这些新的细胞系将支持对欧洲流行的各种病毒,细菌和原生动物蜱传疾病的研究。
Tick cell lines are important tools for research on ticks and the pathogens they transmit. Here, we report the establishment of ten new cell lines from European ticks of the genera Argas, Dermacentor, Hyalomma, Ixodes and Rhipicephalus originating from Germany and Spain. For each cell line, the method used to generate the primary culture, a morphological description of the cells and species confirmation by sequencing of the partial 16S rRNA gene are presented. Further molecular analysis of the two new Ixodes ricinus cell lines and three existing cell lines of the same species revealed genetic variation between cell lines derived from ticks collected in the same or nearby locations. Collectively, these new cell lines will support research into a wide range of viral, bacterial and protozoal tick-borne diseases prevalent in Europe.
DOI: 10.1016/j.ttbdis.2012.05.002
发表时间: 2012-01-01
影响因子: 3.2
作者:
Alberdi, M. Pilar;Dalby, Matthew J.;Bell-Sakyi, Lesley
通讯作者: Bell-Sakyi, Lesley
DOI: 10.1073/pnas.91.21.10034
发表时间: 1994-10-11
影响因子: 11.1
作者:
BLACK, WC;PIESMAN, J
通讯作者: PIESMAN, J
DOI: 10.1017/s003118202000116x
发表时间: 2020-10-01
期刊: PARASITOLOGY
影响因子: 2.4
作者:
Dwuznik, Dorota;Mierzejewska, Ewa Julia;Bajer, Anna
通讯作者: Bajer, Anna
DOI: 10.1016/j.ttbdis.2014.05.003
发表时间: 2014-01-01
影响因子: 3.2
作者:
Estrada-Pena, Agustin;Nava, Santiago;Petney, Trevor
通讯作者: Petney, Trevor
DOI: 10.1016/j.ttbdis.2018.05.015
发表时间: 2018-07
影响因子: 3.2
作者:
Bell-Sakyi L;Darby A;Baylis M;Makepeace BL
通讯作者: Makepeace BL