A Novel In Vitro Assay Using Human iPSC-Derived Sensory Neurons to Evaluate the Effects of External Chemicals on Neuronal Morphology: Possible Implications in the Prediction of Abnormal Skin Sensation.
A Novel In Vitro Assay Using Human iPSC-Derived Sensory Neurons to Evaluate the Effects of External Chemicals on Neuronal Morphology: Possible Implications in the Prediction of Abnormal Skin Sensation.
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一种使用人IPSC衍生的感觉神经元评估外部化学物质对神经元形态的影响的新型体外测定：可能对异常皮肤感觉的预测产生影响。
DOI：
10.3390/ijms221910525
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发表时间：
2021-09-29
影响因子：
5.6
通讯作者：
Takamori K
中科院分区：
文献类型：
--
作者：
Satoh M;Suzuki T;Sakurai T;Toyama S;Kamata Y;Kondo S;Suga Y;Tominaga M;Takamori K
表皮中的神经元形态变化被认为是干性皮肤病中皮肤感觉异常的原因之一。本研究旨在开发一种针对人体皮肤优化的体外模型，以测试导致其恶化的外部因素。人类诱导的多能干细胞来源的感觉神经元（hiPSC-SN）被用作人类感觉神经元的模型。通过观察神经纤维的伸长、水泡（珠状肿胀）的发生率以及烟酰胺单核苷酸腺苷酸转移酶2（NMNAT2）的表达来评估化学物质对这些神经元的影响。接触两种常见的化妆品防腐剂（对羟基苯甲酸甲酯和苯氧乙醇）后，神经纤维长度会增加，但接触苯并[a]芘（表皮中估计浓度的空气污染物）后，神经纤维长度不会增加。此外，接触苯并[a]芘后气泡的发生率增加。然而，神经纤维中 NMNAT2 的表达减少，表明发生了退行性变化。在表皮中使用估计浓度的对羟基苯甲酸甲酯或苯氧乙醇后，没有发现这种变性。这些发现表明，对羟基苯甲酸甲酯和苯氧乙醇促进 hiPSC-SN 中的神经伸长，而苯并[a]芘诱导神经变性。这种改变可能至少部分涉及敏感皮肤的发生和进展。
Neuronal morphological changes in the epidermis are considered to be one of causes of abnormal skin sensations in dry skin-based skin diseases. The present study aimed to develop an in vitro model optimised for human skin to test the external factors that lead to its exacerbation. Human-induced pluripotent stem cell-derived sensory neurons (hiPSC-SNs) were used as a model of human sensory neurons. The effects of chemical substances on these neurons were evaluated by observing the elongation of nerve fibers, incidence of blebs (bead-like swellings), and the expression of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2). The nerve fiber length increased upon exposure to two common cosmetic preservatives—methylparaben and phenoxyethanol—but not to benzo[a]pyrene, an air pollutant at the estimated concentrations in the epidermis. Furthermore, the incidence of blebs increased upon exposure to benzo[a]pyrene. However, there was a decrease in the expression of NMNAT2 in nerve fibers, suggesting degenerative changes. No such degeneration was found after methylparaben or phenoxyethanol at the estimated concentrations in the epidermis. These findings suggest that methylparaben and phenoxyethanol promote nerve elongation in hiPSC-SNs, whereas benzo[a]pyrene induces nerve degeneration. Such alterations may be at least partly involved in the onset and progression of sensitive skin.
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DOI：
10.1096/fj.201902026r
发表时间：
2020-01
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Patil MJ;Salas M;Bialuhin S;Boyd JT;Jeske NA;Akopian AN
通讯作者：
Akopian AN
生物活性化合物在促进神经突生长中的作用。
DOI：
10.3390/molecules17066728
发表时间：
2012-06-04
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
More SV;Koppula S;Kim IS;Kumar H;Kim BW;Choi DK
通讯作者：
Choi DK
DOI：
10.1124/jpet.111.189902
发表时间：
2012-05-01
影响因子：
3.5
作者：
Bianchi, Bruce R.;Zhang, Xu-Feng;Chen, Jun
通讯作者：
Chen, Jun
影响因子：
3.6
作者：
Buhe, Virginie;Vie, Katell;Misery, Laurent
通讯作者：
Misery, Laurent
影响因子：
3.6
作者：
Bray, Eric R.;Cheret, Jeremy;Paus, Ralf
通讯作者：
Paus, Ralf