Increasing the Grain Yield and Grain Protein Content of Common Wheat (Triticum aestivum) by Introducing Missense Mutations in the Q Gene.
Increasing the Grain Yield and Grain Protein Content of Common Wheat (Triticum aestivum) by Introducing Missense Mutations in the Q Gene.
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DOI：
10.3390/ijms231810772
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发表时间：
2022-09-15
影响因子：
5.6
通讯作者：
Qi, Pengfei
中科院分区：
文献类型：
--
作者：
Chen, Qing;Guo, Zhenru;Shi, Xiaoli;Wei, Meiqiao;Fan, Yazhen;Zhu, Jing;Zheng, Ting;Wang, Yan;Kong, Li;Deng, Mei;Cao, Xinyou;Wang, Jirui;Wei, Yuming;Jiang, Qiantao;Jiang, Yunfeng;Chen, Guoyue;Zheng, Youliang;Qi, Pengfei
籽粒产量(GY)和籽粒蛋白质含量(GPC)是小麦育种和生产的重要性状，但它们通常是负相关的。Q基因是栽培小麦中最重要的驯化基因，它影响包括GY和GPC在内的许多性状。Q基因的等位基因变异可能对GY和GPC均有正向影响。因此，我们鉴定了两个新的Q等位基因(Qs1和Qc1-N8)，它们是通过甲烷磺酸乙酯诱变获得的。与野生型Q等位基因相比，QS1在编码第一个AP2结构域的序列中存在错义突变，而Qc1-N8有两个错义突变：一个在编码第二个AP2结构域的序列中，另一个在microRNA172结合位点上。QS1等位基因对GPC和其他加工品质参数没有显著影响，但通过降低千粒重和每穗粒数而对GY产生不利影响。相反，Qc1-N8通过增加千粒重和每穗粒数对GPC和GY有正向影响。因此，我们创造了与小麦育种相关的新种质。为了便于Qc1-N8等位基因在育种中的利用，开发了一种特异的分子标记。此外，我们的发现为通过非转基因方法改良谷类作物提供了有用的新信息。
Grain yield (GY) and grain protein content (GPC) are important traits for wheat breeding and production; however, they are usually negatively correlated. The Q gene is the most important domestication gene in cultivated wheat because it influences many traits, including GY and GPC. Allelic variations in the Q gene may positively affect both GY and GPC. Accordingly, we characterized two new Q alleles (Qs1 and Qc1-N8) obtained through ethyl methanesulfonate-induced mutagenesis. Compared with the wild-type Q allele, Qs1 contains a missense mutation in the sequence encoding the first AP2 domain, whereas Qc1-N8 has two missense mutations: one in the sequence encoding the second AP2 domain and the other in the microRNA172-binding site. The Qs1 allele did not significantly affect GPC or other processing quality parameters, but it adversely affected GY by decreasing the thousand kernel weight and grain number per spike. In contrast, Qc1-N8 positively affected GPC and GY by increasing the thousand kernel weight and grain number per spike. Thus, we generated novel germplasm relevant for wheat breeding. A specific molecular marker was developed to facilitate the use of the Qc1-N8 allele in breeding. Furthermore, our findings provide useful new information for enhancing cereal crops via non-transgenic approaches.
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