The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms including Interference with Auxin Homeostasis.
The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and Inhibits Lepidium sativum Seed Germination via Multiple Mechanisms including Interference with Auxin Homeostasis.
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DOI：
10.3390/ijms23094618
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发表时间：
2022-04-21
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
植物对天然植物毒素的分子响应包括更普遍和化合物特异性的机制。植物毒性的查耳酮和其他类黄酮如何抑制幼苗生长已被广泛研究，但它们如何干扰种子萌发在很大程度上是未知的。二氢查耳酮和推定的化感物质myrigalone A（MyA）抑制种子萌发和幼苗生长。将独行菜种子对MyA的响应的转录组（RNAseq）和激素分析与其他生物活性和非活性化合物进行比较。MyA处理的吸胀种子引发的阶段性诱导的解毒程序，改变赤霉素，顺-（+）-12-氧代植物二烯酸和茉莉酸代谢，并影响激素转运蛋白基因的表达。MyA介导的抑制涉及干扰抗氧化系统，氧化信号传导，水通道蛋白和水的摄取，但不解偶联的氧化磷酸化或对羟基苯丙酮酸双加氧酶的表达/活性。MyA特异性地影响生长素相关信号基因和各种转运蛋白基因的表达，包括生长素转运蛋白（PIN 7，ABCG 37，ABCG 4，WAT 1）。对生长素特异性抑制剂的反应进一步支持了MyA干扰种子萌发过程中生长素稳态的结论。MyA和其他植物毒素的比较分析揭示了特定的调节机制和生长素转运蛋白基因的差异，以干扰生长素稳态。我们的结论是，MyA发挥其植物毒性活性的多种生长素依赖和独立的分子机制。
Molecular responses of plants to natural phytotoxins comprise more general and compound-specific mechanisms. How phytotoxic chalcones and other flavonoids inhibit seedling growth was widely studied, but how they interfere with seed germination is largely unknown. The dihydrochalcone and putative allelochemical myrigalone A (MyA) inhibits seed germination and seedling growth. Transcriptome (RNAseq) and hormone analyses of Lepidium sativum seed responses to MyA were compared to other bioactive and inactive compounds. MyA treatment of imbibed seeds triggered the phased induction of a detoxification programme, altered gibberellin, cis-(+)-12-oxophytodienoic acid and jasmonate metabolism, and affected the expression of hormone transporter genes. The MyA-mediated inhibition involved interference with the antioxidant system, oxidative signalling, aquaporins and water uptake, but not uncoupling of oxidative phosphorylation or p-hydroxyphenylpyruvate dioxygenase expression/activity. MyA specifically affected the expression of auxin-related signalling genes, and various transporter genes, including for auxin transport (PIN7, ABCG37, ABCG4, WAT1). Responses to auxin-specific inhibitors further supported the conclusion that MyA interferes with auxin homeostasis during seed germination. Comparative analysis of MyA and other phytotoxins revealed differences in the specific regulatory mechanisms and auxin transporter genes targeted to interfere with auxin homestasis. We conclude that MyA exerts its phytotoxic activity by multiple auxin-dependent and independent molecular mechanisms.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
7.4
作者：
Bassel, George W.;Fung, Pauline;Cutler, Sean R.
通讯作者：
Cutler, Sean R.
DOI：
10.1073/pnas.1814015116
发表时间：
2019-02-26
影响因子：
11.1
作者：
Feraru, Elena;Feraru, Mugurel I.;Kleine-Vehn, Juergen
通讯作者：
Kleine-Vehn, Juergen
影响因子：
6.5
作者：
Araniti, Fabrizio;Bruno, Leonardo;Abenavoli, Maria Rosa
通讯作者：
Abenavoli, Maria Rosa
影响因子：
7.4
作者：
Cho, Misuk;Lee, Zee-Won;Cho, Hyung-Taeg
通讯作者：
Cho, Hyung-Taeg