Glucose-responsive multifunctional acupuncture needle: A universal SERS detection strategy of small biomolecules in vivo
Glucose-responsive multifunctional acupuncture needle: A universal SERS detection strategy of small biomolecules in vivo
复制标题
葡萄糖响应多功能针灸针：体内小生物分子的通用SERS检测策略
DOI：
10.1039/c2ay25733e
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发表时间：
2012-10
影响因子：
3.1
通讯作者：
Weiping Qian
中科院分区：
文献类型：
--
作者：
Qin Tao;Mingde Guo;Tianyu Yan;Weiping Qian
为了在体内以最小的入侵检测葡萄糖，将葡萄糖氧化酶（GOx，信号转换器）、4-巯基苯甲酸（4-MBA，信号报告者）和微孔聚苯乙烯（PS）包被的sers活性针针（信号放大器）作为集成载体和采样装置，研制了葡萄糖响应多功能针针。在针头上，集成的GOx将葡萄糖转化为葡萄糖酸，改变微环境的pH值，然后集成的4-MBA报告pH变化，间接报告葡萄糖浓度。集成的sers活性材料增强了4-MBA的拉曼信号，提供了葡萄糖浓度的光学读数。SERS检测不依赖于对SERS底物的亲和力和葡萄糖的内在拉曼活性。在微孔ps包被的SERS活性针上整合合适的酶和相应的报告因子，该方法可成为生物体内小分子SERS检测的通用策略。
To detect glucose in vivo with minimal invasion, a glucose-responsive multifunctional acupuncture needle was developed by integrating glucose oxidase (GOx, signal convertor), 4-mercaptobenzoic acid (4-MBA, signal reporter), and a microporous polystyrene (PS)-coated SERS-active acupuncture needle (signal amplifier) which was also used as an integration carrier and sampling device. On the needle, the integrated GOx converted glucose to gluconic acid which changed pH value of the microenvironment and then, the integrated 4-MBA reported the changed pH which indirectly reported the glucose concentration. The integrated SERS-active materials enhanced Raman signal of 4-MBA to provide an optical readout of the glucose concentration. The SERS detection did not depend on the affinity to SERS substrates and intrinsic Raman activity of glucose. Integrating suitable enzymes and corresponding reporters on microporous PS-coated SERS-active needles, the method can become a universal strategy to SERS detection of small biomolecules in vivo.
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影响因子：
7.4
作者：
Yuen, Jonathan M.;Shah, Nilam C.;Walsh, Joseph T., Jr.;Glucksberg, Matthew R.;Van Duyne, Richard P.
通讯作者：
Van Duyne, Richard P.
影响因子：
8.4
作者：
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通讯作者：
Graham, Duncan
影响因子：
7.4
作者：
Hrapovic, S;Luong, JHT
通讯作者：
Luong, JHT
影响因子：
7.4
作者：
G. F. Khan;M. Ohwa;W. Wernet
通讯作者：
G. F. Khan;M. Ohwa;W. Wernet
影响因子：
7.4
作者：
Stone, Nicholas;Faulds, Karen;Matousek, Pavel
通讯作者：
Matousek, Pavel