Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8.
Biodegradation of 2-chloro-4-nitrophenol via a hydroxyquinol pathway by a Gram-negative bacterium, Cupriavidus sp. strain CNP-8.
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革兰氏阴性细菌 Cupriavidus sp 菌株 CNP-8 通过羟基喹啉途径生物降解 2-氯-4-硝基苯酚
DOI：
10.1186/s13568-018-0574-7
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发表时间：
2018-03-20
期刊：
影响因子：
3.7
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Min J;Wang J;Chen W;Hu X
从农药污染土壤中分离到的Cupriavidus sp.菌株CNP-8能够利用2-氯-4-硝基苯酚（2C4NP）作为碳、氮和能量的唯一来源，同时释放亚硝酸盐和氯离子。可以降低2 c4np在温度20 - 40°C和pH值从5到10,和降低2 c4np高达1.6毫米。动力学试验表明,生物降解2 c4np跟着霍尔丹底物抑制模型,与最大比生长速率(μmax) 0.148 /小时,一半饱和常数0.022毫米(Ks)和底物抑制常数(Ki)的0.72毫米。应变CNP-8提出了降低2 c4np hydroxyquinol(1、2、4-benzenetriol BT)开环衬底。菌株CNP-8的2C4NP分解代谢途径与其他革兰氏阴性2C4NP利用菌不同。酶促实验表明，启动2C4NP分解代谢的单加氧酶与先前报道的2C4NP单加氧酶相比，具有不同的底物特异性。毛细管实验表明，菌株CNP-8对2C4NP表现出代谢依赖性的趋化反应，最适浓度为0.5 mM，趋化指数最大为37.5。此外，微观研究表明，菌株CNP-8，特别是预诱导细胞，能够快速地从2C4NP污染的土壤中去除2C4NP。考虑到菌株CNP-8对pH和温度波动的适应性以及对2C4NP的良好降解效率，它可能是一个有希望的候选菌株，用于2C4NP污染位点的生物修复。本文的在线版本（10.1186/s13568-018-0574-7）包含补充材料，授权用户可使用。
Cupriavidus sp. strain CNP-8 isolated from a pesticide-contaminated soil was able to utilize 2-chloro-4-nitrophenol (2C4NP) as a sole source of carbon, nitrogen and energy, together with the release of nitrite and chloride ions. It could degrade 2C4NP at temperatures from 20 to 40 °C and at pH values from 5 to 10, and degrade 2C4NP as high as 1.6 mM. Kinetics assay showed that biodegradation of 2C4NP followed Haldane substrate inhibition model, with the maximum specific growth rate (μmax) of 0.148/h, half saturation constant (Ks) of 0.022 mM and substrate inhibition constant (Ki) of 0.72 mM. Strain CNP-8 was proposed to degrade 2C4NP with hydroxyquinol (1,2,4-benzenetriol, BT) as the ring-cleavage substrate. The 2C4NP catabolic pathway in strain CNP-8 is significant from those reported in other Gram-negative 2C4NP utilizers. Enzymatic assay indicated that the monooxygenase initiating 2C4NP catabolism had different substrates specificity compared with previously reported 2C4NP monooxygenations. Capillary assays showed that strain CNP-8 exhibited metabolism-dependent chemotactic response toward 2C4NP at the optimum concentration of 0.5 mM with a maximum chemotaxis index of 37.5. Furthermore, microcosm studies demonstrated that strain CNP-8, especially the pre-induced cells, could remove 2C4NP rapidly from the 2C4NP-contaminated soil. Considering its adaptability to pH and temperature fluctuations and great degradation efficiency against 2C4NP, strain CNP-8 could be a promising candidate for the bioremediation of 2C4NP-contaminated sites. The online version of this article (10.1186/s13568-018-0574-7) contains supplementary material, which is available to authorized users.
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