Effect of inoculation of Burkholderia sp. strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil.
Effect of inoculation of Burkholderia sp. strain SJ98 on bacterial community dynamics and para-nitrophenol, 3-methyl-4-nitrophenol, and 2-chloro-4-nitrophenol degradation in soil.
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接种伯克霍尔德杆菌SJ98对土壤中细菌群落动态及对硝基苯酚、3-甲基-4-硝基苯酚和2-氯-4-硝基苯酚降解的影响
DOI：
10.1038/s41598-017-06436-0
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发表时间：
2017-07-20
影响因子：
4.6
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Min J;Wang B;Hu X
对硝基苯酚（PNP）、3-甲基-4-硝基苯酚（3M4NP）和2-氯-4-硝基苯酚（2C4NP）是高毒性化合物，已造成严重的环境问题。在人工污染土壤中接种具有降解PNP、3M4NP和2C4NP能力的Burkholderia sp. SJ98菌株，并定量进行生物修复。与未接种处理相比，接种处理中所有硝基酚的降解速度加快。本地细菌能够降解PNP，但不能降解3M4NP和2C4NP。针对分解代谢基因pnpA的Real-time PCR结果显示，菌株SJ98在孵育期间水平保持稳定。高通量测序结果显示，污染和生物增强都影响了细菌群落结构。生物强化似乎保护了Kineosporia， Nitrososphaera和Schlesneria免受硝基苯酚的抑制，并导致nononomuraea， Kribbella和Saccharopolyspora的丰度急剧增加。在污染处理和生物强化处理中，热孢菌、放线菌和链霉菌的相对丰度显著增加；这表明这些细菌可能与硝基酚的降解有直接关系。据我们所知，这是第一个使用生物增强同时去除PNP、3M4NP和2C4NP的报道。本研究为硝基酚污染土壤的生物修复提供了有价值的见解。
para-Nitrophenol (PNP), 3-methyl-4-nitrophenol (3M4NP), and 2-chloro-4-nitrophenol (2C4NP) are highly toxic compounds that have caused serious environmental issues. We inoculated an artificially contaminated soil with Burkholderia sp. strain SJ98, which has the ability to degrade PNP, 3M4NP, and 2C4NP, and quantified bioremediation. There was accelerated degradation of all nitrophenols in inoculated treatments compared to the un-inoculated treatments. The indigenous bacteria were able to degrade PNP, but not 3M4NP or 2C4NP. Real-time PCR targeting the catabolic gene pnpA showed that levels of strain SJ98 remained stable over the incubation period. High-throughput sequencing revealed that both contamination and bioaugmentation influenced the bacterial community structure. Bioaugmentation seemed to protect Kineosporia, Nitrososphaera, and Schlesneria from nitrophenol inhibition, as well as led to a sharp increase in the abundance of Nonomuraea, Kribbella, and Saccharopolyspora. There was a significant increase in the relative abundances of Thermasporomyces, Actinomadura, and Streptomyces in both contaminated and bioaugmented treatments; this indicated that these bacteria are likely directly related to nitrophenol degradation. To our knowledge, this is the first report of the simultaneous removal of PNP, 3M4NP, and 2C4NP using bioaugmentation. This study provides valuable insights into the bioremediation of soils contaminated with nitrophenols.
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影响因子：
3.6
作者：
Laha, S;Petrova, KP
通讯作者：
Petrova, KP
影响因子：
2.2
作者：
Liman, Recep
通讯作者：
Liman, Recep
影响因子：
4.2
作者：
Festa, Sabrina;Macchi, Marianela;Coppotelli, Bibiana M.
通讯作者：
Coppotelli, Bibiana M.
影响因子：
4.4
作者：
Lessner, DJ;Johnson, GR;Gibson, DT
通讯作者：
Gibson, DT
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob