Integrative approach reveals composition of endoparasitoid wasp venoms.
Integrative approach reveals composition of endoparasitoid wasp venoms.
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DOI：
10.1371/journal.pone.0064125
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Schlenke TA
中科院分区：
文献类型：
--
作者：
Goecks J;Mortimer NT;Mobley JA;Bowersock GJ;Taylor J;Schlenke TA
果蝇及其内寄生蜂是研究寄主免疫应答与寄生虫毒力机制相互作用的一个新的模式系统。在这个系统中，黄蜂使用不同的毒液鸡尾酒来抑制保守的苍蝇细胞包裹反应。虽然众多的遗传工具允许详细描述苍蝇的免疫基因，缺乏黄蜂基因组信息阻碍了寄生虫方面的相互作用的表征。在这里，我们使用高通量的核酸和氨基酸测序方法来描述两个相关的果蝇内寄生蜂的毒液与不同的感染策略，Leptopilina boulardi和L。异型瘤使用RNA-seq，我们组装和定量的转录物序列的文库从雌性黄蜂蛹。接下来，我们使用质谱法对来自解剖的毒腺腔的肽进行测序。然后，我们映射的肽光谱数据对腹部转录组，以确定一组推定的毒液基因的每个黄蜂物种。我们的方法捕获了先前在L.通过传统的cDNA克隆方法以及许多新的毒液基因，随后通过RT-PCR，blast比较和分泌信号序列搜索的组合进行验证。总共发现129种蛋白质包含L.结果表明，该菌含有176种蛋白质。异瘤毒液我们在L中发现了显著的重叠。boulardi和L.异瘤蛛毒液的组成，但也有明显的差异，可能是其独特的感染策略。我们的联合转录组蛋白质组学方法的endoparasitoid黄蜂毒液是普遍适用于任何非基因组测序的生物体的功能蛋白质子集的鉴定。
The fruit fly Drosophila melanogaster and its endoparasitoid wasps are a developing model system for interactions between host immune responses and parasite virulence mechanisms. In this system, wasps use diverse venom cocktails to suppress the conserved fly cellular encapsulation response. Although numerous genetic tools allow detailed characterization of fly immune genes, lack of wasp genomic information has hindered characterization of the parasite side of the interaction. Here, we use high-throughput nucleic acid and amino acid sequencing methods to describe the venoms of two related Drosophila endoparasitoids with distinct infection strategies, Leptopilina boulardi and L. heterotoma. Using RNA-seq, we assembled and quantified libraries of transcript sequences from female wasp abdomens. Next, we used mass spectrometry to sequence peptides derived from dissected venom gland lumens. We then mapped the peptide spectral data against the abdomen transcriptomes to identify a set of putative venom genes for each wasp species. Our approach captured the three venom genes previously characterized in L. boulardi by traditional cDNA cloning methods as well as numerous new venom genes that were subsequently validated by a combination of RT-PCR, blast comparisons, and secretion signal sequence search. Overall, 129 proteins were found to comprise L. boulardi venom and 176 proteins were found to comprise L. heterotoma venom. We found significant overlap in L. boulardi and L. heterotoma venom composition but also distinct differences that may underlie their unique infection strategies. Our joint transcriptomic-proteomic approach for endoparasitoid wasp venoms is generally applicable to identification of functional protein subsets from any non-genome sequenced organism.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
3.4
作者：
Asara, John M.;Christofk, Heather R.;Cantley, Lewis C.
通讯作者：
Cantley, Lewis C.
影响因子：
4.8
作者：
Colinet, Dominique;Cazes, Dominique;Poirie, Marylene
通讯作者：
Poirie, Marylene
影响因子：
2.9
作者：
Colinet, Dominique;Dubuffet, Aurore;Poirie, Marylene
通讯作者：
Poirie, Marylene
影响因子：
4
作者：
Fauvarque, Marie-Odile;Williams, Michael J.
通讯作者：
Williams, Michael J.