Survivin delta Ex3 overexpression in thyroid malignancies.
Survivin delta Ex3 overexpression in thyroid malignancies.
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DOI：
10.1371/journal.pone.0100534
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ruchała M
中科院分区：
文献类型：
--
作者：
Waligórska-Stachura J;Andrusiewicz M;Sawicka-Gutaj N;Biczysko M;Jankowska A;Kubiczak M;Czarnywojtek A;Wrotkowska E;Ruchała M
甲状腺癌的发病率在过去几十年中显著增加，是最常见的内分泌恶性肿瘤类型。甲状腺癌的许多因素被研究为预后不良的独立预测因素。本研究的目的是评估生存素表达-BIRC 5及其剪接变体：生存素δ Ex 3和生存素2B在良性和恶性甲状腺结节。来自一组50名患者的甲状腺组织样品，包括：29名甲状腺癌患者（包括髓样、乳头状、滤泡和未分化型），以及来自21名非癌性甲状腺组织患者（包括：11名良性甲状腺病变和10名健康甲状腺样品）。采用定量RT-PCR分析survivin基因表达和评估剪接变异体水平。与良性病变和健康甲状腺样本相比，在甲状腺恶性结节中检测到生存素基因BIRC 5的表达水平在统计学上显著较高。此外，不同阶段肿瘤（pT 1、pT 3和pT 4）中生存素相对表达的比较揭示了在pT 3/pT 4的肿瘤组织中BIRC 5转录物的量高得多。生存素在良性甲状腺结节和健康甲状腺组织中的表达差异无统计学意义。重要的是，生存素δ Ex 3剪接变异体的高表达率是甲状腺癌的特征。结果提示，Survivin，尤其是Survivin δ Ex 3剪接变异体的过表达是甲状腺恶性肿瘤的特征性表达。
Thyroid cancer incidence has increased significantly during the past decades and is the most common type of endocrine malignancy. Many factors in thyroid cancers were studied as independent predictors of a poor prognosis. The objective of the study was to evaluate survivin expression – BIRC5 and its splice variants: survivin delta Ex3 and survivin 2B in benign and malignant thyroid nodules. Thyroid tissues samples from a group of 50 patients consisting of: 29 patients with thyroid cancers (including medullary, papillary, follicular and undifferentiated types), as well as from 21 patients with non-cancerous thyroid tissues (including: 11 benign thyroid lesions and 10 healthy thyroid samples). The analysis of the survivin gene expression and evaluation of the level of splice variants were performed using quantitative RT-PCR. A statistically significant higher level of expression of survivin gene – BIRC5 was detected in thyroid malignant nodules, when compared with benign lesions and healthy thyroid samples. Moreover, the comparison of survivin relative expression in different staged tumors (pT1, pT3, and pT4) revealed a much higher amount of BIRC5 transcripts in tumor tissues of pT3/pT4. The comparison of survivin expression between benign thyroid nodules and healthy thyroid did not reveal significant differences. Importantly, high expression rate of the survivin delta Ex3 splice variant characterized thyroid carcinomas. The results suggest that survivin, especially survivin delta Ex3 splice variant being overexpress, is a characteristic feature of thyroid malignancy.
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影响因子：
5.4
作者：
Chen Z;Liu N;Zhu G;Dralle H;Hoang-Vu C
通讯作者：
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影响因子：
8.8
作者：
Grabowski, P;Kühnel, T;Mühr-Wilkenshoff, F;Heine, B;Stein, H;Höpfner, M;Germer, CT;Scherübl, H
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Scherübl, H
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作者：
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作者：
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通讯作者：
Wang, Xiu-Yun
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4
作者：
Hart CD;De Boer RH
通讯作者：
De Boer RH