PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora.
PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora.
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Apple的PR基因：响应引起者和接种Erwinia amylovora的识别和表达。
DOI：
10.1186/1471-2229-6-23
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发表时间：
2006-10-09
影响因子：
5.3
通讯作者：
Beer, Steven V.
中科院分区：
文献类型：
--
作者：
Bonasera, Jean M.;Kim, Jihyun F.;Beer, Steven V.
在过去的十年里，人们已经做了大量的工作来剖析植物防御信号通路的分子基础，即系统获得性抗性(SAR)。大部分工作都是在拟南芥等模式物种上进行的，很少有人关注木本植物。然而，在所研究的物种范围内，该途径的组成部分似乎是高度保守的。在本研究中，我们试图确定苹果SAR途径的下游成分作为其激活的标记。我们从苹果中鉴定出3个致病相关基因PR-2、PR-5和PR-8，它们在接种苹果病原菌淀粉欧文氏杆菌后被诱导，但在草本植物中用已知的SAR激发子处理不能在苹果幼芽中诱导。根据与模式草本植物已知PR-1基因的序列相似性，我们还从苹果中鉴定出三个PR-1类似基因：PR-1a、PR-1b和PR-1c。接种淀粉芽孢杆菌或用激发子处理都不能诱导PR-1类基因的表达；然而，每种基因都表现出不同的表达模式。对苹果的4个PR基因进行了部分特征分析。来自苹果的PR-1a、PR-2、PR-5和PR-8不是苹果幼枝SAR的标记。通过对GenBank中保存的苹果EST进行电子计算机分析，鉴定出另外两个类似PR-1的基因。PR-1a、PR-1b和PR-1c不参与苹果幼芽的防御反应或SAR；这一结论不同于先前对苹果幼苗的报道。
In the past decade, much work has been done to dissect the molecular basis of the defence signalling pathway in plants known as Systemic Acquired Resistance (SAR). Most of the work has been carried out in model species such as Arabidopsis, with little attention paid to woody plants. However within the range of species examined, components of the pathway seem to be highly conserved. In this study, we attempted to identify downstream components of the SAR pathway in apple to serve as markers for its activation. We identified three pathogenesis related (PR) genes from apple, PR-2, PR-5 and PR-8, which are induced in response to inoculation with the apple pathogen, Erwinia amylovora, but they are not induced in young apple shoots by treatment with known elicitors of SAR in herbaceous plants. We also identified three PR-1-like genes from apple, PR-1a, PR-1b and PR-1c, based solely on sequence similarity to known PR-1 genes of model (intensively researched) herbaceous plants. The PR-1-like genes were not induced in response to inoculation with E. amylovora or by treatment with elicitors; however, each showed a distinct pattern of expression. Four PR genes from apple were partially characterized. PR-1a, PR-2, PR-5 and PR-8 from apple are not markers for SAR in young apple shoots. Two additional PR-1-like genes were identified through in-silico analysis of apple ESTs deposited in GenBank. PR-1a, PR-1b and PR-1c are not involved in defence response or SAR in young apple shoots; this conclusion differs from that reported previously for young apple seedlings.
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影响因子：
56.9
作者：
DELANEY, TP;UKNES, S;RYALS, J
通讯作者：
RYALS, J
影响因子：
5.1
作者：
Komjanc, M;Festi, S;De Lorenzo, G
通讯作者：
De Lorenzo, G
影响因子：
3.5
作者：
LAWTON, KA;BECK, J;RYALS, J
通讯作者：
RYALS, J
影响因子：
2.1
作者：
Loon, L. C. van;Pierpoint, W. S.;Van Loon, L. C.
通讯作者：
Van Loon, L. C.
影响因子：
1.8
作者：
Gau, AE;Koutb, M;Kloppstech, K
通讯作者：
Kloppstech, K