Minichromosome maintenance gene family: potential therapeutic targets and prognostic biomarkers for lung squamous cell carcinoma.
Minichromosome maintenance gene family: potential therapeutic targets and prognostic biomarkers for lung squamous cell carcinoma.
复制标题
微染色体维持基因家族：肺鳞状细胞癌的潜在治疗靶点和预后生物标志物
DOI：
10.18632/aging.204399
复制
发表时间：
2022-11-28
期刊：
影响因子：
5.2
通讯作者：
Ou, Chunlin
中科院分区：
文献类型：
--
作者：
Yang, Xuejie;Wang, Chunrong;Nie, Hui;Zhou, Jianhua;He, Xiaoyun;Ou, Chunlin
小染色体维持（MCM）基因家族由10个成员组成，在真核生物DNA复制中起关键作用，并与许多肿瘤的发生和进展有关。然而，MCM家族是否与肺鳞状细胞癌（LUSC）有关尚不清楚。在这项研究中，我们进行了生物信息学分析，以确定MCM基因在LUSC患者中的作用。我们还评估了他们的差异基因表达、预后相关性、DNA甲基化、基因改变的功能富集和免疫调节。根据肿瘤免疫估计资源数据库，MCM2-10 mRNA在LUSC组织中的表达升高。根据基因表达谱交互分析数据库，MCM2-8和MCM10在LUSC组织中显著上调，所有mcm的蛋白水平在LUSC组织中均升高。此外，在MCM家族成员中，MCM3和MCM7的表达与LUSC患者预后的相关性最强。为了阐明MCM家族的作用和机制，我们进行了京都基因与基因组百科全书和基因本体富集研究。我们发现MCM家族成员的表达模式与浸润性免疫细胞之间存在显著的相关性。总之，我们的研究结果提高了对MCM家族成员在LUSC中的异常表达的理解。这些发现证明了MCM家族作为LUSC诊断和预后的治疗靶点和生物标志物的潜力。
The minichromosome maintenance (MCM) gene family comprises of ten members with key roles in eukaryotic DNA replication and are associated with the occurrence and progression of many tumors. However, whether the MCM family contributes to lung squamous cell carcinoma (LUSC) is unclear. In this study, we performed bioinformatic analysis to identify the roles of MCM genes in patients with LUSC. We also evaluated their differential gene expression, prognostic correlation, DNA methylation, functional enrichment of genetic alterations, and immunomodulation. According to the Tumor Immune Estimation Resource database, the expression of MCM2-10 mRNA was elevated in LUSC tissues. According to the Gene Expression Profiling Interactive Analysis database, MCM2–8 and MCM10 were considerably upregulated in LUSC tissues, and protein levels of all MCMs were increased in LUSC tissues. In addition, among the MCM family members, the expression of MCM3 and MCM7 showed the strongest correlation with the prognoses of patients with LUSC. To clarify the role and mechanisms of the MCM family, Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment studies were performed. We detected a significant correlation between the expression patterns of MCM family members and infiltrating immune cells. In conclusion, our results improve the understanding of the aberrant expression of MCM family members in LUSC. These findings demonstrate the potential of the MCM family as therapeutic targets and biomarkers for the diagnosis and prognosis of LUSC.
登录
查看更多内容
DOI：
10.1016/j.jtho.2017.03.010
发表时间：
2017-06
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
--
作者：
Faruki H;Mayhew GM;Serody JS;Hayes DN;Perou CM;Lai-Goldman M
通讯作者：
Lai-Goldman M
SMC家族在肝细胞癌中的临床意义及综合分析
影响因子：
3.9
作者：
Nie H;Wang Y;Yang X;Liao Z;He X;Zhou J;Ou C
通讯作者：
Ou C
MCM2-7复制解旋酶：一个有希望的化学治疗靶标。
影响因子：
--
作者：
Simon NE;Schwacha A
通讯作者：
Schwacha A
影响因子：
7.5
作者：
Fei L;Xu H
通讯作者：
Xu H
影响因子：
11.2
作者：
Li T;Fan J;Wang B;Traugh N;Chen Q;Liu JS;Li B;Liu XS
通讯作者：
Liu XS