Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA.
Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA.
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DOI：
10.1016/j.jviromet.2010.07.012
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发表时间：
2010-10
影响因子：
3.1
通讯作者：
Damon, Inger K.
中科院分区：
文献类型：
--
作者：
Li, Yu;Zhao, Hui;Wilkins, Kimberly;Hughes, Christine;Damon, Inger K.
正痘病毒猴痘（MPXV）形成两个不同的分支：MPXV刚果盆地分支病毒在刚果盆地流行，人类疾病通常表现出与离散的普通天花相似的症状，在未接种疫苗的人群中病死率约为10%; MPXV西非分支病毒已在西非分离，似乎引起不太严重，人与人之间传染性较低的疾病。最近，美国和苏丹分别报告了由MPXV西非和刚果盆地株引起的猴痘暴发。这些事件证明了该病毒利用新宿主并在全球范围内出现的能力和趋势;它还强调了对MPXV诊断的需要，特别是区分刚果盆地和西非猴痘毒株的能力。在本研究中，报告了三种基于TaqMan探针技术的新实时PCR检测方法：MPXV西非特异性检测、刚果盆地菌株特异性检测和MPXV通用检测。新的检测方法在多平台和各种PCR试剂盒的验证研究中表现出良好的特异性和灵敏度，并将提高猴痘感染与其他皮疹疾病的快速检测和区分。
Orthopoxvirus monkeypox (MPXV) forms two distinct clades: the MPXV Congo Basin clade viruses are endemic in the Congo Basin, human illness typically presents with symptoms similar to discrete, ordinary smallpox and has a case fatality rate of approximately 10% in unvaccinated populations; the MPXV West African clade viruses have been isolated in West Africa and appear to cause a less severe, and less inter-human transmissible disease. Recently, monkeypox outbreaks were reported in US and Sudan caused by MPXV West African and Congo Basin strains respectively. These events demonstrated the ability and trend of the virus to exploit new hosts and emerge globally; it also emphasizes the need for the diagnosis of MPXV, especially the ability to distinguish between Congo Basin and West African monkeypox strains. In this study, three new real-time PCR assays based on TaqMan probe technology were reported: the MPXV West African specific, Congo Basin strain specific and MPXV generic assays. The new assays demonstrated good specificity and sensitivity in the validation study with multiple platforms and various PCR reagent kits, and will improve the rapid detection and differentiation of monkeypox infections from other rash illness.
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影响因子：
3.1
作者：
Meyer, H;Ropp, SL;Esposito, JJ
通讯作者：
Esposito, JJ
影响因子：
158.5
作者：
Reed, KD;Melski, JW;Damon, IK
通讯作者：
Damon, IK
影响因子：
3.8
作者：
Likos, AM;Sammons, SA;Damon, IK
通讯作者：
Damon, IK
DOI：
10.4269/ajtmh.2007.76.757
发表时间：
2007-04-01
影响因子：
3.3
作者：
Hutson, Christina L.;Lee, Kemba N.;Regnery, Russell L.
通讯作者：
Regnery, Russell L.
影响因子：
8.8
作者：
Li, Yu;Olson, Victoria A.;Laue, Thomas;Laker, Miriam T.;Damon, Inger K.
通讯作者：
Damon, Inger K.