Inhibition of angiogenesis, tumour growth and experimental metastasis of human fibrosarcoma cells HT1080 by a multimeric form of the laminin sequence Tyr-Ile-Gly-Ser-Arg (YIGSR).
Inhibition of angiogenesis, tumour growth and experimental metastasis of human fibrosarcoma cells HT1080 by a multimeric form of the laminin sequence Tyr-Ile-Gly-Ser-Arg (YIGSR).
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DOI：
10.1038/bjc.1996.102
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发表时间：
1996-03
影响因子：
8.8
通讯作者：
Sugioka, Y
中科院分区：
文献类型：
--
作者：
Iwamoto, Y;Nomizu, M;Yamada, Y;Ito, Y;Tanaka, K;Sugioka, Y
研究了由层粘连蛋白16个YIGSR序列组成的多聚体Ac-Y16对HT1080人纤维肉瘤细胞的实验性转移、血管生成和肿瘤生长的影响。联合注射Ac-Y16 0.5 mg/只小鼠。使用HT1080细胞可抑制100%的肺定植，而每只小鼠0.5 mg单体Ac-YIGSR-NH2(AcY1)可抑制94%的肺定植。AC-Y16在体内对肿瘤细胞无直接细胞毒作用。通过计算新生血管面积和称重肿瘤重量来评价多肽对血管生成和肿瘤生长的影响。HT1080细胞基底膜提取物及其多肽移植到裸鼠体内。AC-Y16皮下注射0.5 mg/只。HT1080对血管生成和肿瘤生长的抑制率分别为92%(P<；0.05)和76%(P<；0.05)，而0.5 mg/只Ac-YIGSR-NH2(Ac-Y1)对血管生成和肿瘤生长的抑制率分别为40%(P<；0.05)和9%(P>；0.05)。从这些数据可以推断，Ac-Y16的抗肿瘤作用可能是通过抑制血管生成来实现的。腹腔注射Ac-Y16还能有效抑制HT1080细胞的血管生成、肿瘤生长和肺定植。结论：含多聚体YIGSR的多肽Ac-Y16对血管生成、肿瘤生长和实验性转移的抑制作用比单体形式更强，并且在ip、iv时具有活性。和S.C.
A multimeric peptide, Ac-Y16, consisting of 16 YIGSR sequences from laminin was evaluated for its effect on experimental metastasis, angiogenesis and tumour growth of HT1080 human fibrosarcoma cells. Co-injection of 0.5 mg per mouse of Ac-Y16 i.v. with HT 1080 cells inhibited lung colonisation by 100%, whereas 0.5 mg per mouse of monomeric Ac-YIGSR-NH2(AcY1) inhibited by 94%. Ac-Y16 did not show any direct cytotoxicity in tumour cells in vivo. The effect of the peptides on angiogenesis and tumour growth respectively were evaluated by counting areas of neovessels and weighing tumours after the s.c. implantation of HT1080 cells with basement membrane extracts and the peptide into nude mice. Co-injection of 0.5 mg per mouse of AC-Y16 s.c. with HT1080 cells inhibited angiogenesis and tumour growth by 92% (P<0.05) and 76% (P<0.05) respectively, whereas 0.5 mg per mouse of monomeric Ac-YIGSR-NH2(Ac-Y1) inhibited angiogenesis and tumour growth by 40% (P<0.05) and 9% (P>0.05) respectively. It can be inferred from these data that anti-tumour effects of Ac-Y16 are likely to result from anti-angiogenesis. Intraperitoneal administration of Ac-Y16 was also effective in inhibiting angiogenesis, tumour growth and lung colonisation of HT1080 cells. It was concluded that the multimeric YIGSR-containing peptide, Ac-Y16, inhibits angiogenesis, tumour growth and experimental metastasis more than the monomeric form and that it is active when administered i.p., iv. and s.c.
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影响因子：
4
作者：
IWAMOTO, Y;FUJITA, Y;SUGIOKA, Y
通讯作者：
SUGIOKA, Y
影响因子：
2.9
作者：
KLEINMAN, HK;MCGARVEY, ML;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
5.6
作者：
IWAMOTO, Y;GRAF, J;YAMADA, Y
通讯作者：
YAMADA, Y
DOI：
10.1016/0141-8130(89)90049-4
发表时间：
1989-04-01
影响因子：
8.2
作者：
MURATA, J;SAIKI, I;NISHI, N
通讯作者：
NISHI, N
影响因子：
64.5
作者：
GRANT, DS;TASHIRO, KI;KLEINMAN, HK
通讯作者：
KLEINMAN, HK