The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad.
The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad.
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DOI：
10.1007/s00784-013-1166-1
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发表时间：
2014-11
影响因子：
3.4
通讯作者：
Kotani, Junichiro
中科院分区：
文献类型：
--
作者：
Kishimoto, Naotaka;Momota, Yoshihiro;Hashimoto, Yoshiya;Tatsumi, Shinichi;Ando, Kayoko;Omasa, Takeshi;Kotani, Junichiro
关键词：
本研究的目的是评价和比较颊脂垫（BFP）去分化脂肪（DFAT）细胞和脂肪干细胞（ASCs）的成骨分化能力。我们从一位接受口腔颌面外科手术的患者的BFP中分离人DFAT细胞和ASCs，然后通过流式细胞术分析其细胞表面抗原。然后，将细胞在成骨培养基中培养14天。通过骨特异性碱性磷酸酶（BAP）、骨钙素（OCN）、钙沉积和茜素红染色来评价两种细胞类型的成骨分化能力。ASCs和DFAT细胞CD90和CD105阳性，CD11b、CD34和CD45阴性。DFAT细胞培养物中的BAP（第3天和第7天）、OCN（第14天）和钙沉积（第7天和第14天）显著高于ASC培养物中的那些。DFAT细胞培养物中的茜素红染色区域，其指示矿化基质沉积，比ASC培养物中的茜素红染色更强烈。ASCs和DFAT细胞的细胞表面抗原趋于相似。此外，人DFAT细胞的成骨分化能力高于BFP来源的ASC。从BFP中分离DFAT细胞具有美学优势，因为BFP可以通过口腔获得而不损伤外部体表。因此，我们认为，从BFP的DFAT细胞是一个理想的细胞来源，骨组织工程。
The purpose of this study was to evaluate and compare the osteoblastic differentiation ability of dedifferentiated fat (DFAT) cells and adipose stem cells (ASCs) from the buccal fat pad (BFP). We isolated human DFAT cells and ASCs from the BFP of a patient who underwent oral and maxillofacial surgery and then analyzed their cell surface antigens by flow cytometry. Then, the cells were cultured in osteogenic medium for 14 days. Measurement of bone-specific alkaline phosphatase (BAP), osteocalcin (OCN), and calcium deposition and alizarin red staining were performed to evaluate the osteoblastic differentiation ability of both cell types. ASCs and DFAT cells were positive for CD90 and CD105 and negative for CD11b, CD34, and CD45. BAP (days 3 and 7), OCN (day 14), and calcium deposition (days 7 and 14) within DFAT cell cultures were significantly higher than those in ASC cultures. The alizarin red-stained area in DFAT cell cultures, which indicates mineralized matrix deposition, was stained more strongly than that in ASC cultures. The cell surface antigens of ASCs and DFAT cells tend to be similar. Furthermore, the osteoblastic differentiation ability of human DFAT cells is higher than that of ASCs from the BFP. Isolation of DFAT cells from the BFP has an esthetic advantage because the BFP can be obtained via the oral cavity without injury to the external body surface. Therefore, we consider that DFAT cells from the BFP are an ideal cell source for bone tissue engineering.
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影响因子：
3
作者：
Farre-Guasch, Elisabet;Marti-Pages, Carles;Casals, Nuria
通讯作者：
Casals, Nuria
影响因子：
3.3
作者：
Zuk, PA;Zhu, M;Hedrick, MH
通讯作者：
Hedrick, MH
DOI：
10.1016/s0266-4356(97)90680-x
发表时间：
1997-04-01
影响因子：
1.8
作者：
MartinGranizo, R;Naval, L;Diaz, F
通讯作者：
Diaz, F
影响因子：
2.8
作者：
Gao, Qian;Zhao, Lili;Yang, Gongshe
通讯作者：
Yang, Gongshe
影响因子：
4.3
作者：
El Haddad, Sally A.;El Razzak, Mona Y. Abd;El Shall, Mohammad
通讯作者：
El Shall, Mohammad