Cisplatin-loaded hollow gold nanoparticles for laser-triggered release.
Cisplatin-loaded hollow gold nanoparticles for laser-triggered release.
复制标题
DOI：
10.1186/s12645-018-0041-9
复制
发表时间：
2018
影响因子：
5.7
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Xiong C;Lu W;Zhou M;Wen X;Li C
空心金纳米粒子（HGNP）暴露于近红外（NIR）光产生光热效应，可以引发潜在的生物医学应用的各种生物效应。然而，激光触发药物释放的机理还没有被研究过。将三肽Ac-Glu-Glu-Cys-NH 2（Ac-EEC）直接连接到HGNP的表面。然后将EEC-HGNP缀合物与顺铂Pt（II）络合以得到Ac-EEC（Pt）-HGNP。叶酸通过硫辛酸封端的聚乙二醇接头（F-PEG-TA）引入到Ac-EEC-HGNP的金表面，然后与Pt（II）络合，得到F-Ac-EEC（Pt）-HGNP。激光处理是用15 ns脉冲激光以10 Hz的重复频率进行的。通过电感耦合等离子体质谱法对释放的Pt（II）进行定量，并通过液相色谱-质谱法对释放的含Pt物质的性质进行表征。MTT法检测细胞毒性。Pt（II）通过两种模式从Ac-EEC（Pt）-HGNP中释放：（1）通过与氯离子的逆配体交换反应持续释放和（2）在NIR激光照射下通过三肽接头和Au表面之间的Au-S键断裂快速释放。与激光处理组合的纳米构建体的叶酸（F）缀合物F-Ac-EEC（Pt）-HGNP在孵育24小时后显示出比单独的F-Ac-ECC（Pt）-HGNP对叶酸过表达的人表皮样癌KB细胞的细胞死亡率显著更大的影响。这些结果表明，HGNP的光热性质可用于双模态光热治疗和NIR激光触发的铂基化疗。
Hollow gold nanoparticles (HGNPs) exposed to near-infrared (NIR) light yield photothermal effects that can trigger a variety of biological effects for potential biomedical applications. However, the mechanism of laser-triggered drug release has not been studied before. A tripeptide Ac-Glu-Glu-Cys-NH2 (Ac-EEC) was directly linked to the surface of HGNPs. The EEC-HGNPs conjugate was then complexed with cisplatin Pt(II) to give Ac-EEC(Pt)-HGNPs. Folic acid was introduced to the gold surface of Ac-EEC-HGNPs through a thioctic acid-terminated polyethylene glycol linker (F-PEG-TA) followed by complexation with Pt(II) to give F-Ac-EEC(Pt)-HGNPs. Laser treatment was instituted with a 15-ns pulsed laser at a repetition rate of 10 Hz. The released Pt(II) was quantified by inductively coupled plasma mass spectroscopy, and the nature of the released Pt-containing species was characterized by liquid chromatography–mass spectroscopy. The cytotoxicity was studied using the MTT assay. Pt(II) was released from Ac-EEC(Pt)-HGNPs via two modes: (1) sustained release through an inverse ligand exchange reaction with chloride ions and (2) rapid release through cleavage of the Au–S bond between the tripeptide linker and Au surface upon NIR laser irradiation. The folate (F) conjugate of the nanoconstruct, F-Ac-EEC(Pt)-HGNPs, in combination with laser treatment showed a significantly greater effect on cell mortality against folate-overexpressing human epidermoid carcinoma KB cells than F-Ac-ECC(Pt)-HGNPs alone after 24 h of incubation. These results demonstrate that the photothermal property of HGNPs can be used for dual-modality photothermal therapy and NIR laser-triggered platinum-based chemotherapy.
登录
查看更多内容
聚乙二醇包被的空心金纳米球的药代动力学、清除率和生物安全性
影响因子：
10
作者：
You J;Zhou J;Zhou M;Liu Y;Robertson JD;Liang D;Van Pelt C;Li C
通讯作者：
Li C
影响因子：
11.2
作者：
Lu W;Zhang G;Zhang R;Flores LG 2nd;Huang Q;Gelovani JG;Li C
通讯作者：
Li C
靶向空心金纳米球介导的光声成像和光热消融治疗对神经胶质瘤原位小鼠异种移植模型的影响。
影响因子：
11.2
作者：
Lu W;Melancon MP;Xiong C;Huang Q;Elliott A;Song S;Zhang R;Flores LG 2nd;Gelovani JG;Wang LV;Ku G;Stafford RJ;Li C
通讯作者：
Li C
影响因子：
14
作者：
Lu, Wei;Huang, Qian;Ku, Geng;Wen, Xiaoxia;Zhou, Min;Guzatov, Dmitry;Brecht, Peter;Su, Richard;Oraevsky, Alexander;Wang, Lihong V.;Li, Chun
通讯作者：
Li, Chun
影响因子：
15
作者：
Cheng, Kai;Peng, Sheng;Xu, Chenjie;Sun, Shouheng
通讯作者：
Sun, Shouheng