Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation.
Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation.
复制标题
毛囊及其囊肿中的桥粒芯糖蛋白同种型表达与角化类型和分化程度相关。
DOI：
10.1046/j.1523-1747.2003.12234.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
G. Cotsarelis
中科院分区：
文献类型：
--
作者：
Hong Wu;J. Stanley;G. Cotsarelis
在复层鳞状上皮如表皮内，桥粒芯糖蛋白通常以分化特异性方式表达。与表皮相似，毛囊根据其分化水平分为不同的细胞类型。隆突中相对未分化的干细胞可以生成表皮、皮脂腺和毛球基质细胞。后者产生至少六种不同的细胞类型，当它们沿着毛干和内根鞘向上移动时，它们会角质化。在这里，我们研究的桥粒芯糖蛋白同种型，桥粒芯糖蛋白1，2和3在皮肤上皮细胞的表达模式，并发现桥粒芯糖蛋白1和2的表达与毛囊内的定义的人群的分化状态。桥粒芯糖蛋白2由皮肤上皮的最低分化细胞高度表达，包括胎儿和成人的毛囊隆起、球基质细胞和外根鞘的基底层。相反，桥粒芯糖蛋白1定义更多的分化细胞群，并在表皮基底上细胞，内根鞘，和外根鞘的最内层中表达。我们发现桥粒芯糖蛋白3的表达模式与不同类型的角化相关。在毛囊中的毛鞘角化区域和从这些区域产生的囊肿中，桥粒芯糖蛋白3在整个外根鞘和囊肿壁的所有层中表达。在表皮样角化的区域，如漏斗部和表皮包涵体囊肿中，桥粒芯糖蛋白3的表达主要限于基底层。我们的结论是，桥粒芯糖蛋白的表达模式定义车厢内的皮肤上皮细胞在类似的分化状态，并揭示了这些车厢之间的分化层次。
Within stratified squamous epithelia, such as the epidermis, desmogleins are generally expressed in a differentiation-specific manner. Similar to the epidermis, the hair follicle is compartmentalized into a hierarchy of cell types based on their level of differentiation. Relatively undifferentiated stem cells in the bulge can generate epidermis, sebaceous gland, and hair bulb matrix cells. The latter give rise to at least six different cell types that keratinize as they move up the hair shaft and inner root sheath. Here, we examined expression patterns of the desmoglein isotypes, desmogleins 1, 2, and 3 in the cutaneous epithelium, and discovered that desmoglein 1 and 2 expression correlated with the state of differentiation of defined populations within the hair follicle. Desmoglein 2 was highly expressed by the least differentiated cells of the cutaneous epithelium, including the hair follicle bulge of the fetus and adult, bulb matrix cells, and basal layer of the outer root sheath. In contrast, desmoglein 1 defined more differentiated cell populations, and was expressed in epidermal suprabasal cells, the inner root sheath, and the innermost layers of the outer root sheath. We found that the expression pattern of desmoglein 3 correlated with different types of keratinization. In areas of trichilemmal keratinization in the follicle, and in cysts arising from these areas, desmoglein 3 was expressed throughout all layers of the outer root sheath and cyst wall. In areas of epidermal-like keratinization, such as in the infundibulum and in epidermal inclusion cysts, desmoglein 3 expression was limited mainly to the basal layer. We conclude that desmoglein expression patterns define compartments of cells in similar states of differentiation within the cutaneous epithelium, and reveal a hierarchy of differentiation among these compartments.
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影响因子：
4
作者：
P. J. Koch;M. Mahoney;G. Cotsarelis;K. Rothenberger;R. Lavker;J. Stanley
通讯作者：
P. J. Koch;M. Mahoney;G. Cotsarelis;K. Rothenberger;R. Lavker;J. Stanley
影响因子：
6.5
作者：
Morris, RJ;Potten, CS
通讯作者：
Potten, CS
影响因子：
6.5
作者：
AKIYAMA, M;DALE, BA;HOLBROOK, KA
通讯作者：
HOLBROOK, KA
DOI：
10.1038/sj.jidsp.5640233
发表时间：
1999-12-01
影响因子：
--
作者：
Lyle, S;Christofidou-Solomidou, M;Cotsarelis, G
通讯作者：
Cotsarelis, G