Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.
Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.
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Piwi与核孔处的染色质相互作用，并混合结合果蝇卵巢体细胞中的核转录本。
DOI：
10.1093/nar/gkx355
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发表时间：
2017-07-27
影响因子：
14.9
通讯作者：
Shevelyov YY
中科院分区：
文献类型：
--
作者：
Ilyin AA;Ryazansky SS;Doronin SA;Olenkina OM;Mikhaleva EA;Yakushev EY;Abramov YA;Belyakin SN;Ivankin AV;Pindyurin AV;Gvozdev VA;Klenov MS;Shevelyov YY
Piwi与Piwi相互作用RNA（piRNA）形成复合物，触发果蝇卵巢中转座因子（TE）的转录沉默，从而确保基因组稳定性。为此，Piwi必须扫描基因和TE的新生转录物，以获得与piRNA的互补性。这种扫描的机制目前尚不清楚。在这里，我们报告了果蝇卵巢体细胞中多个Piwi相互作用的染色体结构域的DamID-seq作图。这些结构域与核孔复合物（NPC）的基因组区域显著重叠。因此，Piwi与NPC Elys的组分以及已知与NPC相互作用的RNA转录和输出复合物的Xmas-2亚基共免疫沉淀。然而，只有一个小的Piwi部分有短暂的访问DNA在核孔。重要的是，尽管36%的蛋白质编码基因与Piwi相互作用结构域重叠，并且RNA免疫沉淀结果表明Piwi与许多基因和TE核转录物混杂结合，但根据现有数据，Piwi不会沉默这些基因，这可能是由于piRNA及其转录物之间缺乏完美的碱基配对。
Piwi in a complex with Piwi-interacting RNAs (piRNAs) triggers transcriptional silencing of transposable elements (TEs) in Drosophila ovaries, thus ensuring genome stability. To do this, Piwi must scan the nascent transcripts of genes and TEs for complementarity to piRNAs. The mechanism of this scanning is currently unknown. Here we report the DamID-seq mapping of multiple Piwi-interacting chromosomal domains in somatic cells of Drosophila ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes (NPCs). Accordingly, Piwi was coimmunoprecipitated with the component of NPCs Elys and with the Xmas-2 subunit of RNA transcription and export complex, known to interact with NPCs. However, only a small Piwi fraction has transient access to DNA at nuclear pores. Importantly, although 36% of the protein-coding genes overlap with Piwi-interacting domains and RNA-immunoprecipitation results demonstrate promiscuous Piwi binding to numerous genic and TE nuclear transcripts, according to available data Piwi does not silence these genes, likely due to the absence of perfect base-pairing between piRNAs and their transcripts.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
13.8
作者：
Czech B;Hannon GJ
通讯作者：
Hannon GJ
影响因子：
10.5
作者：
Kopytova, Daria V.;Orlova, Anastasija V.;Georgieva, Sofia G.
通讯作者：
Georgieva, Sofia G.
影响因子：
4.6
作者：
Luo, Shengzhan D.;Shi, Guang W.;Baker, Bruce S.
通讯作者：
Baker, Bruce S.