The interaction between PRRSV and the late gestation pig fetus.
The interaction between PRRSV and the late gestation pig fetus.
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DOI：
10.1016/j.virusres.2010.09.001
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发表时间：
2010-12
期刊：
影响因子：
5
通讯作者：
Rowland RR
中科院分区：
文献类型：
--
作者：
Rowland RR
猪繁殖与呼吸综合征病毒（PRRSV）在妊娠晚期通过胎盘感染胎儿。在妊娠109-112天（临分娩前）回收的胎仔组织中测量病毒复制和细胞因子反应。恢复时，感染母鼠的感染和非感染胎仔均出现明显的大体解剖异常。病毒分离和免疫组织化学鉴定胸腺为病毒复制的主要部位。稳态RT-PCR扩增的炎症，Th 1和Th 2细胞因子，显示感染胎儿组织中IFN-γ和TNF-α mRNA的升高，这对应于血清中升高的细胞因子蛋白，而不是羊水。在淋巴结的增生反应中发现了诱导免疫的进一步证据，其包括占据CD w 75 + B细胞的生发中心的发育。总的来说，这些数据支持免疫活性胎儿能够启动抗病毒反应的观点，这种反应在感染的胎儿中是区室化的。此外，胎儿病理可能不是病毒在胎儿中复制的直接结果。
Porcine reproductive and respiratory syndrome virus (PRRSV) crosses the placenta during late gestation and productively infects the fetus. Virus replication and cytokine responses were measured in tissues of fetuses recovered at 109–112 days of gestation, just prior to parturition. At the time of recovery, gross anatomical abnormalities were evident in both infected and non-infected fetuses from the infected dams. Virus isolation and immunohistochemistry identified the thymus as the primary site of virus replication. Steady state RT-PCR amplification of inflammatory, Th1 and Th2 cytokines, showed elevated IFN-γ and TNF-α mRNAs in tissues from infected fetuses, which corresponded to elevated cytokine proteins in serum but not amniotic fluid. Further evidence for induction of immunity was found in the hyperplastic response of lymph nodes, which included the development of germinal centers occupied CDw75+ B cells. Collectively, these data support the notion that the immunocompetent fetus is capable of initiating an antiviral response, which is compartmentalized within the infected fetus. Furthermore, fetal pathology may not be a direct result of virus replication in the fetus.
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DOI：
10.1016/j.ejogrb.2009.06.028
发表时间：
2009-11-01
影响因子：
2.6
作者：
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通讯作者：
Guindi, Wael E. L.
影响因子：
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作者：
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影响因子：
1.5
作者：
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CHLADEK, D
影响因子：
3.4
作者：
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通讯作者：
Martal, J
影响因子：
1.5
作者：
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通讯作者：
Halbur, PG