RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteins.
RBP Image Database: A resource for the systematic characterization of the subcellular distribution properties of human RNA binding proteins.
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DOI：
10.1093/nar/gkac971
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发表时间：
2023-01-06
影响因子：
14.9
通讯作者：
Lecuyer, Eric
中科院分区：
文献类型：
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作者：
Bouvrette, Louis Philip Benoit;Wang, Xiaofeng;Boulais, Jonathan;Kong, Jian;Syed, Easin Uddin;Blue, Steven M.;Zhan, Lijun;Olson, Sara;Stanton, Rebecca;Wei, Xintao;Yee, Brian;Van Nostrand, Eric L.;Fu, Xiang-Dong;Burge, Christopher B.;Graveley, Brenton R.;Yeo, Gene W.;Lecuyer, Eric
RNA结合蛋白(RNAbindingProteins，RBPs)是基因表达的中枢调节因子，参与RNA代谢的各个方面。因此，它们在细胞生理学和疾病病因学中起着关键作用。由于转录后基因表达的不同步骤往往发生在细胞的特定区域，包括核或细胞质位置，因此确定限制性商业惯例的亚细胞分布特性是评估其潜在功能的重要步骤。在这里，我们展示了RBP图像数据库，这是一个详细说明了301个RBPs在人类HepG2和HeLa细胞系中的亚细胞定位特征的资源，基于系统的免疫荧光研究的结果，该研究使用了高度验证的RBP抗体集合和一组针对特定细胞器和亚细胞结构的12个标记。RBP图像数据库的独特功能包括：(I)托管每个RBP标记对的全面代表性图像，以及∼250,000张显微镜图像；(Ii)手动管理的注释术语控制词汇表，详细说明每个因素的本地化特征；以及(Iii)用户友好的界面，允许按目标或注释快速查询数据。RBP图像数据库可在https://rnabiology.ircm.qc.ca/RBPImage/.上免费获取
RNA binding proteins (RBPs) are central regulators of gene expression implicated in all facets of RNA metabolism. As such, they play key roles in cellular physiology and disease etiology. Since different steps of post-transcriptional gene expression tend to occur in specific regions of the cell, including nuclear or cytoplasmic locations, defining the subcellular distribution properties of RBPs is an important step in assessing their potential functions. Here, we present the RBP Image Database, a resource that details the subcellular localization features of 301 RBPs in the human HepG2 and HeLa cell lines, based on the results of systematic immuno-fluorescence studies conducted using a highly validated collection of RBP antibodies and a panel of 12 markers for specific organelles and subcellular structures. The unique features of the RBP Image Database include: (i) hosting of comprehensive representative images for each RBP-marker pair, with ∼250,000 microscopy images; (ii) a manually curated controlled vocabulary of annotation terms detailing the localization features of each factor; and (iii) a user-friendly interface allowing the rapid querying of the data by target or annotation. The RBP Image Database is freely available at https://rnabiology.ircm.qc.ca/RBPImage/.
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影响因子：
14.9
作者：
Cook KB;Kazan H;Zuberi K;Morris Q;Hughes TR
通讯作者：
Hughes TR
DOI：
10.1073/pnas.82.19.6576
发表时间：
1985-01-01
影响因子：
11.1
作者：
MAHER, PA;PASQUALE, EB;SINGER, SJ
通讯作者：
SINGER, SJ
影响因子：
16
作者：
Caudron-Herger, Maiwen;Rusin, Scott F.;Diederichs, Sven
通讯作者：
Diederichs, Sven
DOI：
10.1093/database/baw035
发表时间：
2016-04-07
影响因子：
5.8
作者：
Giudice, Girolamo;Sanchez-Cabo, Fatima;Lara-Pezzi, Enrique
通讯作者：
Lara-Pezzi, Enrique
影响因子：
64.8
作者：
通讯作者：
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