Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes.
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DOI：
10.1016/j.immuni.2013.08.007
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发表时间：
2013-09-19
期刊：
影响因子：
32.4
通讯作者：
Randolph GJ
中科院分区：
文献类型：
--
作者：
Jakubzick C;Gautier EL;Gibbings SL;Sojka DK;Schlitzer A;Johnson TE;Ivanov S;Duan Q;Bala S;Condon T;van Rooijen N;Grainger JR;Belkaid Y;Ma'ayan A;Riches DW;Yokoyama WM;Ginhoux F;Henson PM;Randolph GJ
据认为，单核细胞在离开血液后迅速分化为巨噬细胞或树突状细胞（DC）。在这里，我们已经表明，Ly-6C+单核细胞组成型运输到皮肤，肺和淋巴结（LN）。在gnotobiotic小鼠中进入未受影响。静息肺和LN中的单核细胞具有与血液单核细胞相似的基因表达谱，但是由单核细胞与内皮细胞相互作用诱导的有限数量的基因的转录升高，包括环加氧酶-2（考克斯-2）和主要组织相容性复合物II类（MHC II）。联体共生，溴脱氧尿苷（BrdU）脉冲追踪分析，和鼻内滴注示踪剂表明，而不是有助于居民巨噬细胞在肺中，招募内源性单核细胞获得抗原运输到引流LN，一个功能冗余与DC虽然分化为DC没有发生。因此，单核细胞可以进入稳态非淋巴器官并再循环至淋巴结，而不分化为巨噬细胞或DC，从而修正了长期以来认为单核细胞默认成为组织驻留巨噬细胞的观点。
It is thought that monocytes rapidly differentiate to macrophages or dendritic cells (DCs) upon leaving blood. Here we have shown that Ly-6C+ monocytes constitutively trafficked into skin, lung, and lymph nodes (LNs). Entry was unaffected in gnotobiotic mice. Monocytes in resting lung and LN had similar gene expression profiles to blood monocytes, but elevated transcripts of a limited number of genes including cyclo-oxygenase-2 (COX-2) and major histocompatibility complex class II (MHC II), induced by monocyte interaction with endothelium. Parabiosis, bromodoxyuridine (BrdU) pulse-chase analysis, and intranasal instillation of tracers indicated that instead of contributing to resident macrophages in the lung, recruited endogenous monocytes acquired antigen for carriage to draining LNs, a function redundant with DCs though differentiation to DCs did not occur. Thus, monocytes can enter steady state non-lymphoid organs and recirculate to LNs without differentiation to macrophages or DCs, revising a long-held view that monocytes become tissue-resident macrophages by default.
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影响因子：
4.4
作者：
Sallusto, Federica;Lanzavecchia, Antonio
通讯作者：
Lanzavecchia, Antonio
单核吞噬细胞的起源和动力学。
影响因子：
15.3
作者：
van Furth, R;Cohn, Z A
通讯作者：
Cohn, Z A
影响因子：
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作者：
Eberlein, Jens;Nguyen, Tom T.;Homann, Dirk
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影响因子：
30.5
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--
影响因子：
32.4
作者：
Serbina, NV;Salazar-Mather, TP;Pamer, EG
通讯作者：
Pamer, EG