Organization of multisynaptic inputs to the dorsal and ventral dentate gyrus: retrograde trans-synaptic tracing with rabies virus vector in the rat.
Organization of multisynaptic inputs to the dorsal and ventral dentate gyrus: retrograde trans-synaptic tracing with rabies virus vector in the rat.
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DOI：
10.1371/journal.pone.0078928
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Iijima T
中科院分区：
文献类型：
--
作者：
Ohara S;Sato S;Tsutsui K;Witter MP;Iijima T
行为学、解剖学和基因表达研究表明，背侧海马和腹侧海马在空间认知、情绪和压力方面存在功能分离。本研究利用重组狂犬病毒载体的逆行突触示踪，研究了大鼠背侧和腹侧齿状回（DG）多突触输入的差异。将载体注射到DG背侧或腹侧3天后，在嗅内皮层、内侧隔、对角带和乳上核中存在单突触神经元标记，每个都直接投射到DG。与之前的追踪研究一样，在这些区域可以看到与背侧和腹侧DG相关的地形模式。感染后第5天，这些区域的标记神经元增多，皮层和皮层下区域也可见到标记神经元，包括梨状和内侧前额叶皮质、梨状内核、屏状核、皮质杏仁核、中缝核内侧、缰核内侧、脚间核和外侧隔。与单突触标记区域一样，标记神经元的地形分布在大多数这些双突触标记区域是明显的。这些数据表明，皮层和皮层下输入到背侧和腹侧DG是通过平行的突触通路传递的。背侧和腹侧DG的这种二阶输入差异可能有助于海马沿背侧轴的功能分化。
Behavioral, anatomical, and gene expression studies have shown functional dissociations between the dorsal and ventral hippocampus with regard to their involvement in spatial cognition, emotion, and stress. In this study we examined the difference of the multisynaptic inputs to the dorsal and ventral dentate gyrus (DG) in the rat by using retrograde trans-synaptic tracing of recombinant rabies virus vectors. Three days after the vectors were injected into the dorsal or ventral DG, monosynaptic neuronal labeling was present in the entorhinal cortex, medial septum, diagonal band, and supramammillary nucleus, each of which is known to project to the DG directly. As in previous tracing studies, topographical patterns related to the dorsal and ventral DG were seen in these regions. Five days after infection, more of the neurons in these regions were labeled and labeled neurons were also seen in cortical and subcortical regions, including the piriform and medial prefrontal cortices, the endopiriform nucleus, the claustrum, the cortical amygdala, the medial raphe nucleus, the medial habenular nucleus, the interpeduncular nucleus, and the lateral septum. As in the monosynaptically labeled regions, a topographical distribution of labeled neurons was evident in most of these disynaptically labeled regions. These data indicate that the cortical and subcortical inputs to the dorsal and ventral DG are conveyed through parallel disynaptic pathways. This second-order input difference in the dorsal and ventral DG is likely to contribute to the functional differentiation of the hippocampus along the dorsoventral axis.
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