Otx2 enhances transdifferentiation of Müller cells‐derived retinal stem cells into photoreceptor‐like cells
Otx2 enhances transdifferentiation of Müller cells‐derived retinal stem cells into photoreceptor‐like cells
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Otx2 增强 Müller 细胞衍生的视网膜干细胞向光感受器样细胞的转分化
DOI：
10.1111/jcmm.13995
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Xiaobo Xia
中科院分区：
文献类型：
--
作者：
Yu Xiong;Hongpei Ji;Zhipeng You;Fei Yao;Rongrong Zhou;Weitao Song;Xiaobo Xia
Retinal Müller glial cells have the potential of neurogenic retinal progenitor cells, and could reprogram into retinal‐specific cell types such as photoreceptor cells. How to promote the differentiation of Müller cells into photoreceptor cells represents a promising therapy strategy for retinal degeneration diseases. This study aimed to enhance the transdifferentiation of rat Müller cells‐derived retinal stem cells (MC‐RSCs) into photoreceptor‐like cells and explore the signalling mechanism. We dedifferentiated rat Müller cells into MC‐RSCs which were infected with Otx2 overexpression lentivirus or control. The positive rate of photoreceptor‐like cells among MC‐RSCs treated with Otx2 overexpression lentivirus was significantly higher compared to control. Furthermore, pre‐treatment with Crx siRNA, Nrl siRNA, or GSK‐3 inhibitor SB‐216763 reduced the positive rate of photoreceptor‐like cells among MC‐RSCs treated with Otx2 overexpression lentivirus. Finally, Otx2 induced photoreceptor precursor cells were injected into subretinal space ofN‐methyl‐N‐nitrosourea induced rat model of retinal degeneration and partially recovered retinal degeneration in the rats. In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target for gene therapy of retinal degenerative diseases.
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DOI：
10.1111/gtc.12127
发表时间：
2014-03
期刊：
Genes to cells : devoted to molecular & cellular mechanisms
影响因子：
--
作者：
Seko Y;Azuma N;Ishii T;Komuta Y;Miyamoto K;Miyagawa Y;Kaneda M;Umezawa A
通讯作者：
Umezawa A
影响因子：
64.8
作者：
Pearson, R. A.;Barber, A. C.;Rizzi, M.;Hippert, C.;Xue, T.;West, E. L.;Duran, Y.;Smith, A. J.;Chuang, J. Z.;Azam, S. A.;Luhmann, U. F. O.;Benucci, A.;Sung, C. H.;Bainbridge, J. W.;Carandini, M.;Yau, K. -W.;Sowden, J. C.;Ali, R. R.
通讯作者：
Ali, R. R.
DOI：
10.1002/dvdy.24375
发表时间：
2016-07
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Hamon A;Roger JE;Yang XJ;Perron M
通讯作者：
Perron M
影响因子：
16.6
作者：
Zhong, Xiufeng;Gutierrez, Christian;Xue, Tian;Hampton, Christopher;Vergara, M. Natalia;Cao, Li-Hui;Peters, Ann;Park, Tea Soon;Zambidis, Elias T.;Meyer, Jason S.;Gamm, David M.;Yau, King-Wai;Canto-Soler, M. Valeria
通讯作者：
Canto-Soler, M. Valeria
DOI：
10.1016/j.trsl.2013.11.011
发表时间：
2014-04
期刊：
Translational research : the journal of laboratory and clinical medicine
影响因子：
--
作者：
Yu H;Vu TH;Cho KS;Guo C;Chen DF
通讯作者：
Chen DF