Differential intra-abdominal adipose tissue profiling in obese, insulin-resistant women.
Differential intra-abdominal adipose tissue profiling in obese, insulin-resistant women.
复制标题
肥胖，耐胰岛素女性的腹腔内脂肪组织分析。
DOI：
10.1007/s11695-009-9949-9
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发表时间：
2009-11
期刊：
影响因子：
2.9
通讯作者：
Tsao, Philip S.
中科院分区：
文献类型：
--
作者：
Liu, Alice;McLaughlin, Tracey;Liu, Teresa;Sherman, Arthur;Yee, Gail;Abbasi, Fahim;Lamendola, Cindy;Morton, John;Cushman, Samuel W.;Reaven, Gerald M.;Tsao, Philip S.
我们最近发现，与肥胖匹配的胰岛素敏感个体相比，胰岛素抵抗（IR）的腹部皮下脂肪组织（SAT）存在差异，包括小脂肪细胞的积累、细胞分化标记物表达的减少以及炎症活动的增加。这项研究的目的是观察 IR 个体 SAT 的这些变化是否存在于网膜内脏脂肪组织 (VAT) 中；在这种情况下，个体被选为 IR，但肥胖程度各不相同。我们比较了接受减肥手术的 IR 个体的细胞大小分布以及 SAT 和 VAT 的遗传标记。通过胰岛素抑制试验，11 名肥胖/病态肥胖女性出现 IR。将脂肪组织手术样本固定在四氧化锇中，通过 Beckman Coulter Multisizer 进行细胞大小分析。对与脂肪细胞分化和炎症相关的基因进行定量实时聚合酶链反应。虽然小细胞的比例和脂肪细胞分化基因的表达在不同储存库之间没有差异，但炎症基因在 VAT 中上调。 SAT 大细胞的直径与 SAT 和 VAT 中小细胞比例的增加高度相关（分别为 r=0.85，p=0.001；r=0.72，p=0.01）。在任一仓库中均未观察到增值税大细胞与细胞大小变量之间的关联。体重指数 (BMI) 对两个仓库中任何变量的影响都可以忽略不计。 IR 女性 VAT 的主要差异特征是炎症活动增加，与 BMI 无关。 SAT 脂肪细胞肥大与两个储存库中增生的关联表明 SAT 可能在调节区域脂肪储存中发挥主要作用。
We recently identified differences in abdominal subcutaneous adipose tissue (SAT) from insulin-resistant (IR) as compared to obesity-matched insulin sensitive individuals, including accumulation of small adipose cells, decreased expression of cell differentiation markers, and increased inflammatory activity. This study was initiated to see if these changes in SAT of IR individuals were present in omental visceral adipose tissue (VAT); in this instance, individuals were chosen to be IR but varied in degree of adiposity. We compared cell size distribution and genetic markers in SAT and VAT of IR individuals undergoing bariatric surgery. Eleven obese/morbidly obese women were IR by the insulin suppression test. Adipose tissue surgical samples were fixed in osmium tetroxide for cell size analysis via Beckman Coulter Multisizer. Quantitative real-time polymerase chain reaction for genes related to adipocyte differentiation and inflammation was performed. While proportion of small cells and expression of adipocyte differentiation genes did not differ between depots, inflammatory genes were upregulated in VAT. Diameter of SAT large cells correlated highly with increasing proportion of small cells in both SAT and VAT (r=0.85, p= 0.001; r=0.72, p=0.01, respectively). No associations were observed between VAT large cells and cell size variables in either depot. The effect of body mass index (BMI) on any variables in both depots was negligible. The major differential property of VAT of IR women is increased inflammatory activity, independent of BMI. The association of SAT adipocyte hypertrophy with hyperplasia in both depots suggests a primary role SAT may have in regulating regional fat storage.
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DOI：
10.1152/ajpendo.2001.281.3.e586
发表时间：
2001-09-01
影响因子：
5.1
作者：
Livingston, EH;Lee, S
通讯作者：
Lee, S
影响因子：
--
作者：
FAUST, IM;JOHNSON, PR;HIRSCH, J
通讯作者：
HIRSCH, J
影响因子：
--
作者：
McLaughlin, Tracey;Abbasi, Fahim;Reaven, Gerald
通讯作者：
Reaven, Gerald
影响因子：
5.8
作者：
Bruun, JM;Lihn, AS;Richelsen, B
通讯作者：
Richelsen, B
影响因子：
4.8
作者：
Fain, JN;Madan, AK;Bahouth, SW
通讯作者：
Bahouth, SW