Cloning and characterization of porcine 4Ig-B7-H3: a potent inhibitor of porcine T-cell activation.
Cloning and characterization of porcine 4Ig-B7-H3: a potent inhibitor of porcine T-cell activation.
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DOI：
10.1371/journal.pone.0021341
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Chen W;Hou Z;Li C;Xiong S;Liu H
B7超家族成员在抗原特异性体液和细胞介导的免疫应答的启动和维持期间共刺激淋巴细胞增殖。B7-H3（CD 276）是新近发现的B7超家族成员。已经显示B7-H3在调节人和小鼠中的T细胞应答中起重要作用，但是不知道在猪物种中是否存在人或鼠B7-H3的对应物。通过在NCBI数据库中与人b7-h3进行blast搜索、RT-PCR和3′端RACE，克隆了猪4 ig-b7-h3基因。蛋白质序列分析表明，该基因编码的蛋白含有4个Ig样结构域，与人4 Ig-B7-H3的同源性为90.88%。斑点杂交和RT-PCR结果表明，B7-H3在猪组织中广泛分布，主要以2 Ig-B7-H3和4 Ig-B7-H3两种亚型存在，其中4 Ig-B7-H3占优势。我们进一步证明，猪4 Ig-B7-H3能够抑制通过TCR途径活化的猪T细胞的增殖和细胞因子产生，类似于人B7-H3。我们克隆了猪4 Ig-B7-H3基因，并证明了猪4 Ig-B7-H3作为T细胞免疫应答的负调节剂。
Members of the B7 superfamily costimulate the proliferation of lymphocytes during the initiation and maintenance of antigen-specific humoral and cell-mediated immune responses. B7-H3 (CD276) is a newly identified member of the B7 superfamily. It has been shown that B7-H3 plays a significant role in regulating T cell response in humans and mice, but it is not known whether a counterpart of human or murine B7-H3 exists in porcine species. We cloned the porcine 4ig-b7-h3 gene using a blast search at the NCBI database with human b7-h3, RT-PCR and 3′-terminus RACE. Protein sequence analysis showed that the protein encoded by this gene contained 4Ig-like domains and was 90.88% identical with human 4Ig-B7-H3. Results of Dot-blot hybridization and RT-PCR showed that B7-H3 was broadly distributed in porcine tissues mainly as two isoforms, 2Ig-B7-H3 and 4Ig-B7-H3, of which 4Ig-B7-H3 was dominant. We further demonstrated that porcine 4Ig-B7-H3 was able to inhibit the proliferation and cytokine production of porcine T cells activated through the TCR pathway, similar to human B7-H3. We cloned the porcine 4ig-b7-h3 gene and demonstrated that the porcine 4Ig-B7-H3 serves as a negative regulator for the T-cell immune response.
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