SPC-P1: a pathogenicity-associated prophage of Salmonella paratyphi C.
SPC-P1: a pathogenicity-associated prophage of Salmonella paratyphi C.
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SPC-P1：丙型副伤寒沙门氏菌致病性相关原噬菌体
DOI：
10.1186/1471-2164-11-729
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发表时间：
2010-12-30
期刊：
影响因子：
4.4
通讯作者：
Liu SL
中科院分区：
文献类型：
--
作者：
Zou QH;Li QH;Zhu HY;Feng Y;Li YG;Johnston RN;Liu GR;Liu SL
背景
丙型副伤寒沙门氏菌是与沙门氏菌一起沿着的少数几种人类适应性病原体之一。伤寒沙门氏菌（S.甲型副伤寒和S.副伤寒B引起伤寒，但目前还不清楚这些细菌是否通过相同或不同的致病机制引起疾病。值得注意的是，这些伤寒因子具有不同的大基因组插入序列，其可以编码不同的致病因子。在此之前，我们在S.副伤寒C RKS 4594，并想知道它是否可能参与细菌的致病性。
结果
我们分析了SPC-P1的序列，发现它是一种诱导型噬菌体，其总G+C含量为47.24%，与大多数沙门氏菌如P22和ST 64 T相似，但显著低于RKS 4594染色体的平均值52.16%。电子显微镜显示短尾噬菌体颗粒非常类似于类杆状噬菌体CUS-3。为了评估其在致病性中的作用，我们将S.将不具有该前噬菌体的丙型副伤寒菌株CN 13/87用溶原化的CN 13/87感染小鼠。与无噬菌体的野生型CN 13/87相比，溶原化的CN 13/87在相当低的感染剂量下表现出显著增加的毒力，并在小鼠中引起多器官损伤。
结论
SPC-P1对S.因此，该原噬菌体可能参与了人类伤寒的发病机制。SPC-P1的遗传和功能分析可能有助于研究现存伤寒因子的致病进化，为阐明伤寒因子的致病决定因素提供特别的帮助。
Background
Salmonella paratyphi C is one of the few human-adapted pathogens along with S. typhi, S. paratyphi A and S. paratyphi B that cause typhoid, but it is not clear whether these bacteria cause the disease by the same or different pathogenic mechanisms. Notably, these typhoid agents have distinct sets of large genomic insertions, which may encode different pathogenicity factors. Previously we identified a novel prophage, SPC-P1, in S. paratyphi C RKS4594 and wondered whether it might be involved in pathogenicity of the bacteria.
Results
We analyzed the sequence of SPC-P1 and found that it is an inducible phage with an overall G+C content of 47.24%, similar to that of most Salmonella phages such as P22 and ST64T but significantly lower than the 52.16% average of the RKS4594 chromosome. Electron microscopy showed short-tailed phage particles very similar to the lambdoid phage CUS-3. To evaluate its roles in pathogenicity, we lysogenized S. paratyphi C strain CN13/87, which did not have this prophage, and infected mice with the lysogenized CN13/87. Compared to the phage-free wild type CN13/87, the lysogenized CN13/87 exhibited significantly increased virulence and caused multi-organ damages in mice at considerably lower infection doses.
Conclusions
SPC-P1 contributes pathogenicity to S. paratyphi C in animal infection models, so it is possible that this prophage is involved in typhoid pathogenesis in humans. Genetic and functional analyses of SPC-P1 may facilitate the study of pathogenic evolution of the extant typhoid agents, providing particular help in elucidating the pathogenic determinants of the typhoid agents.
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影响因子：
2.6
作者：
Popoff, MY;Bockemühl, J;Gheesling, LL
通讯作者：
Gheesling, LL
影响因子：
14.9
作者：
Chiu CH;Tang P;Chu C;Hu S;Bao Q;Yu J;Chou YY;Wang HS;Lee YS
通讯作者：
Lee YS
影响因子：
3.2
作者：
Pedulla, ML;Ford, ME;Casjens, SR
通讯作者：
Casjens, SR
影响因子：
3.2
作者：
Casjens, SR;Gilcrease, EB;Hendrix, RW
通讯作者：
Hendrix, RW
影响因子：
3.2
作者：
Mmolawa, PT;Schmieger, H;Heuzenroeder, MW
通讯作者：
Heuzenroeder, MW