Potential interactions between pericytes and oligodendrocyte precursor cells in perivascular regions of cerebral white matter.
Potential interactions between pericytes and oligodendrocyte precursor cells in perivascular regions of cerebral white matter.
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DOI：
10.1016/j.neulet.2015.04.047
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发表时间：
2015-06-15
影响因子：
2.5
通讯作者：
Arai K
中科院分区：
文献类型：
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作者：
Maki T;Maeda M;Uemura M;Lo EK;Terasaki Y;Liang AC;Shindo A;Choi YK;Taguchi A;Matsuyama T;Takahashi R;Ihara M;Arai K
周细胞镶嵌在基底板内，在脑内血管周围的壁龛中扮演多种角色。近年来，少突胶质前体细胞(OPC)也被报道与脑内皮细胞相关。有没有可能在这个神经胶质血管部位，周细胞和OPC之间也存在潜在的空间和功能上的相互作用？在这里，我们证明了在大脑白质的血管周围区域，周细胞和OPC可以相互附着和支持。免疫组织化学染色显示，在出生后0、60和240d，周细胞和OPC在小鼠白质内定位紧密接触。电子显微镜分析证实，周细胞通过血管周围的基底膜附着在OPC上。在死后的人类大脑中也观察到了这两种细胞类型之间的密切联系。通过体外传代实验评价周细胞与OPC之间的功能相互作用。用周细胞条件培养液处理OPC时，OPC数量增加。同样，当周细胞保持在OPC条件培养液中时，周细胞数量增加。综上所述，我们的数据提示了大脑白质中周细胞和OPC之间潜在的解剖和功能相互作用。
Pericytes are embedded within basal lamina and play multiple roles in the perivascular niche in brain. Recently, oligodendrocyte precursor cells (OPCs) have also been reported to associate with cerebral endothelium. Is it possible that within this gliovascular locus, there may also exist potential spatial and functional interactions between pericytes and OPCs? Here, we demonstrated that in the perivascular region of cerebral white matter, pericytes and OPCs may attach and support each other. Immunostaining showed that pericytes and OPCs are localized in close contact with each other in mouse white matter at postnatal days 0, 60 and 240. Electron microscopic analysis confirmed that pericytes attached to OPCs via basal lamina in the perivascular region. The close proximity between these two cell types was also observed in postmortem human brains. Functional interaction between pericytes and OPCs was assessed by in vitro media transfer experiments. When OPC cultures were treated with pericyte-conditioned media, OPC number increased. Similarly, pericyte number increased when pericytes were maintained in OPC-conditioned media. Taken together, our data suggest a potential anatomical and functional interaction between pericytes and OPCs in cerebral white matter.
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影响因子：
16.2
作者：
Kang, Shin H.;Fukaya, Masahiro;Yang, Jason K.;Rothstein, Jeffrey D.;Bergles, Dwight E.
通讯作者：
Bergles, Dwight E.
影响因子：
5.3
作者：
Arai, Ken;Lo, Eng H.
通讯作者：
Lo, Eng H.
影响因子：
2.5
作者：
Hayakawa K;Seo JH;Pham LD;Miyamoto N;Som AT;Guo S;Kim KW;Lo EH;Arai K
通讯作者：
Arai K
影响因子：
23.9
作者：
Karow, Marisa;Sanchez, Rodrigo;Berninger, Benedikt
通讯作者：
Berninger, Benedikt
影响因子：
64.8
作者：
通讯作者：
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