Thermally Driven Membrane Phase Transitions Enable Content Reshuffling in Primitive Cells.
Thermally Driven Membrane Phase Transitions Enable Content Reshuffling in Primitive Cells.
复制标题
热驱动的膜相转变使原始细胞中的含量重新填充。
DOI：
10.1021/jacs.1c06595
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发表时间：
2021-10-13
影响因子：
15
通讯作者：
Bonfio C
中科院分区：
文献类型：
--
作者：
Rubio-Sánchez R;O'Flaherty DK;Wang A;Coscia F;Petris G;Di Michele L;Cicuta P;Bonfio C
在生前环境中可获得的自组装单链两亲分子可能在原始细胞周期的出现中发挥了重要作用。然而，基于益生素脂肪酸的膜对温度和pH的不稳定性似乎表明，原始细胞只能在狭窄的、不波动的环境条件下进行益生相关的过程。在这里，我们认为，由环境波动驱动的膜相变，使具有重新洗牌内容的子代原始细胞的产生成为可能。可逆膜到油的相变解释了基于脂肪酸的小泡在高温下的溶解和伴随而来的原细胞内容物的释放。在低温下，脂肪酸双层重新组装并将重新洗牌的物质包裹在一组新的原细胞中。值得注意的是，我们发现我们的拆卸/重组周期推动了含有功能RNA的原始细胞从父母的非功能隔间中出现。因此，通过利用益生素脂肪酸囊泡的内在不稳定性，我们的结果指出了一种环境驱动的可调的益生菌过程，它支持寡核苷酸和膜组件的释放和重新洗牌，潜在地导致具有优越特征的新一代原细胞。在缺乏原细胞运输机制的情况下，本文提出的环境驱动的拆解/组装周期似乎支持将原细胞内容重新洗牌传递给原始细胞后代，暗示了一种潜在的机制，该机制对启动早期生命形式的达尔文进化很重要。
Self-assembling single-chain amphiphiles available in the prebiotic environment likely played a fundamental role in the advent of primitive cell cycles. However, the instability of prebiotic fatty acid-based membranes to temperature and pH seems to suggest that primitive cells could only host prebiotically relevant processes in a narrow range of nonfluctuating environmental conditions. Here we propose that membrane phase transitions, driven by environmental fluctuations, enabled the generation of daughter protocells with reshuffled content. A reversible membrane-to-oil phase transition accounts for the dissolution of fatty acid-based vesicles at high temperatures and the concomitant release of protocellular content. At low temperatures, fatty acid bilayers reassemble and encapsulate reshuffled material in a new cohort of protocells. Notably, we find that our disassembly/reassembly cycle drives the emergence of functional RNA-containing primitive cells from parent nonfunctional compartments. Thus, by exploiting the intrinsic instability of prebiotic fatty acid vesicles, our results point at an environmentally driven tunable prebiotic process, which supports the release and reshuffling of oligonucleotides and membrane components, potentially leading to a new generation of protocells with superior traits. In the absence of protocellular transport machinery, the environmentally driven disassembly/assembly cycle proposed herein would have plausibly supported protocellular content reshuffling transmitted to primitive cell progeny, hinting at a potential mechanism important to initiate Darwinian evolution of early life forms.
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影响因子：
2.9
作者：
Mariani A;Bonfio C;Johnson CM;Sutherland JD
通讯作者：
Sutherland JD
影响因子：
21.8
作者：
Adamala KP;Martin-Alarcon DA;Guthrie-Honea KR;Boyden ES
通讯作者：
Boyden ES
DOI：
10.1073/pnas.0805086105
发表时间：
2008-09-09
影响因子：
11.1
作者：
Mansy, Sheref S.;Szostak, Jack W.
通讯作者：
Szostak, Jack W.
影响因子：
2.9
作者：
GOOD, NE;WINGET, GD;SINGH, RMM
通讯作者：
SINGH, RMM
影响因子：
4.6
作者：
Kudella, Patrick W.;Preissinger, Katharina;Westerhausen, Christoph
通讯作者：
Westerhausen, Christoph