Estrogen preserves split renal function in a chronic complete unilateral ureteral obstruction animal model.
Estrogen preserves split renal function in a chronic complete unilateral ureteral obstruction animal model.
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DOI：
10.3892/etm.2014.1663
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发表时间：
2014-06
影响因子：
2.7
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Mao S;Xu H;Zou L;Xu G;Wu Z;Ding Q;Jiang H
雌激素可能有助于保护慢性肾病患者的肾功能。本研究探讨了雌激素给药或剥夺是否影响慢性单侧输尿管梗阻 (UUO) 大鼠的肾功能分裂。将 15 只成年雌性 Sprague-Dawley 大鼠随机分为三组。低雌激素组和高雌激素组分别采用雌性去势或腹腔注射雌激素造模，正常雌激素组不予处理。 UUO手术后第2、6和16天通过单光子发射计算机断层扫描（SPECT/CT）对大鼠进行间歇性肾功能分裂[肾小球滤过率（GFR）]检查。常规苏木精和伊红(H&E)染色、免疫组化、病理学检查和电镜检查比较组织学差异。成功建立低雌激素组、正常雌激素组和高雌激素组（P<0.001）。急性期，正常组和高雌激素组对侧健康肾GFR较低雌激素组有更大的代偿性升高（P<0.05）。慢性期梗阻肾GFR持续下降，高雌激素组GFR保存明显好于低雌激素组（P<0.05）。高雌激素组对侧肾脏GFR补偿程度最大（P=0.01），总GFR明显优于高雌激素组（P<0.05）。常规H&E检查显示手术后显着的组织学变化。转化生长因子-β（TGF-β）和α-平滑肌肌动蛋白（α-SMA）免疫组化（IHC）检查显示，低雌激素组肾间质纤维化较正常组和高雌激素组明显（P<0.05）。电子显微镜（EM）检查也区分了各组。总之，雌激素的施用和剥夺显着影响肾功能。雌激素可以保护慢性 UUO 大鼠的肾裂功能 (GFR)。
Estrogen may help to preserve renal function in chronic kidney disease. This study examined whether estrogen administration or deprivation affected the split renal function in rats subjected to chronic unilateral ureteral obstruction (UUO). Fifteen adult female Sprague-Dawley rats were randomly divided into three groups. Low- and high-estrogen groups were modeled by female castration or estrogen intraperitoneal injection, respectively, and the rats in the normal-estrogen group were untreated. Intermittent split renal function [glomerular filtration rate (GFR)] examination was performed on rats on days 2, 6 and 16 after UUO surgery via single-photon emission computed tomography (SPECT/CT). Routine hematoxylin and eosin (H&E) staining, immunohistochemistry, pathology examination and electron microscopy were performed to compare the histological differences. Low-, normal- and high-estrogen groups were successfully established (P<0.001). In the acute stage, the GFR of the contralateral healthy kidney showed a greater compensatory rise in the normal- and high-estrogen groups than in the low-estrogen group (P<0.05). In the chronic stage, the GFR of the obstructed kidney continued to decrease with the GFR of the high-estrogen group being significantly better preserved than that of the low-estrogen group (P<0.05). The GFR of the contralateral kidney compensated to the greatest extent in the high-estrogen group (P=0.01), and the total GFR was significantly superior (P<0.05). Routine H&E examination showed significant histological changes following surgery. The low-estrogen group had significant renal interstitial fibrosis compared with the normal- and high-estrogen groups (P<0.05), as observed by immunohistochemical (IHC) examination of transforming growth factor-β (TGF-β) and α-smooth muscle actin (α-SMA). Electron-microscopic (EM) examination also differentiated between groups. In conclusion, estrogen administration and deprivation significantly affected renal function. Estrogen may preserve the split renal function (GFR) in rats with chronic UUO.
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影响因子：
3.5
作者：
Heiman, Julia R.;Rupp, Heather;Laan, Ellen
通讯作者：
Laan, Ellen
影响因子：
--
作者：
Takahashi, Takeshi;Yamane, Akifumi;Takeuchi, Hideo
通讯作者：
Takeuchi, Hideo
影响因子：
8.8
作者：
Cherikh, W. S.;Young, C. J.;Fan, P. -Y.
通讯作者：
Fan, P. -Y.
DOI：
10.1152/ajprenal.00145.2002
发表时间：
2002-11-01
影响因子：
4.2
作者：
Armando, I;Jezova, M;Saavedra, JM
通讯作者：
Saavedra, JM
DOI：
10.1152/ajprenal.00441.2003
发表时间：
2004-12-01
影响因子：
4.2
作者：
Ito, K;Chen, J;Felsen, D
通讯作者：
Felsen, D